SMCR7/MID49 Polyclonal antibody
SMCR7/MID49 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Mouse, Rat and More (3)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 28718-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | Jurkat cells, mouse heart tissue, rat heart tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
Product Information
28718-1-AP targets SMCR7/MID49 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Reactivity | Human, Mouse, Rat |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SMCR7/MID49 fusion protein Ag30119 相同性解析による交差性が予測される生物種 |
| Full Name | Smith-Magenis syndrome chromosome region, candidate 7 |
| Calculated molecular weight | 454 aa, 49 kDa |
| Observed molecular weight | 49 kDa |
| GenBank accession number | BC014973 |
| Gene symbol | MID49 |
| Gene ID (NCBI) | 125170 |
| RRID | AB_2881198 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SMCR7, also named as MID49, is a 49 kDa mitochondrial outer membrane protein which regulates mitochondrial morphology. SMCR7 can directly recruit the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface. SMCR7 has two isoforms.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SMCR7/MID49 antibody 28718-1-AP | Download protocol |
| IHC protocol for SMCR7/MID49 antibody 28718-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Med Characterisation of cold-induced mitochondrial fission in porcine aortic endothelial cells. | ||
Nat Commun Auranofin targets UBA1 and enhances UBA1 activity by facilitating ubiquitin trans-thioesterification to E2 ubiquitin-conjugating enzymes | ||
Int J Biol Sci Mitochondrial Dysfunction by FADDosome Promotes Gastric Mucosal Injury in Portal Hypertensive Gastropathy |
