Validation Data Gallery

Filter: AllWBIHC

WB analysis using 16413-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16413-1-AP (SMCR7/MID49 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293T using 16413-1-AP
HEK-293T cells were subjected to SDS PAGE followed by western blot with 16413-1-AP (SMCR7/MID49 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HT-1080 using 16413-1-AP
HT-1080 cells were subjected to SDS PAGE followed by western blot with 16413-1-AP (SMCR7/MID49 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HT-1080 using 16413-1-AP
HT-1080 cells were subjected to SDS PAGE followed by western blot with 16413-1-AP (SMCR7/MID49 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HT-1080 using 16413-1-AP
HT-1080 cells were subjected to SDS PAGE followed by western blot with 16413-1-AP (SMCR7/MID49 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 16413-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16413-1-AP (SMCR7/MID49 antibody at dilution of 1:50 (under 10x lens).
IHC staining of human liver cancer using 16413-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16413-1-AP (SMCR7/MID49 antibody at dilution of 1:50 (under 40x lens).
IHC staining of human skeletal muscle using 16413-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16413-1-AP (SMCR7/MID49 antibody at dilution of 1:50 (under 10x lens).
IHC staining of human skeletal muscle using 16413-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16413-1-AP (SMCR7/MID49 antibody at dilution of 1:50 (under 40x lens).

Featured Product
KD/KO Validated

SMCR7/MID49 Polyclonal antibody

SMCR7/MID49 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16413-1-AP

Publications(54)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF, ELISA

Synonyms

MID49, MIEF2, SMCR7

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (54)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293T cells, HepG2 cells, HT-1080 cells
Positive IHC detected in	human liver cancer tissue, human skeletal muscle tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 10 publications below
WB	See 52 publications below
IHC	See 3 publications below
IF	See 3 publications below

Product Information

16413-1-AP targets SMCR7/MID49 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat, monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SMCR7/MID49 fusion protein Ag9531 相同性解析による交差性が予測される生物種
Full Name	Smith-Magenis syndrome chromosome region, candidate 7
Calculated molecular weight	454 aa, 49 kDa
Observed molecular weight	49 kDa
GenBank accession number	BC014973
Gene Symbol	MID49
Gene ID (NCBI)	125170
RRID	AB_2714217
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96C03
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SMCR7, also named as MID49, is a 49 kDa mitochondrial outer membrane protein which regulates mitochondrial morphology. SMCR7 can directly recruit the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface. SMCR7 has two isoforms.

Protocols

Product Specific Protocols
WB protocol for SMCR7/MID49 antibody 16413-1-AP	Download protocol
IHC protocol for SMCR7/MID49 antibody 16413-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human		Science Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division. Authors - Shun Nagashima View Article
mouse	WB	Cell Metab Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells. Authors - Xiuying Zhong View Article
monkey	WB	Nat Cell Biol MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control. Authors - Tim König KO Validated View Article
human	WB	Autophagy Loss of MIEF1/MiD51 confers susceptibility to BAX-mediated cell death and PINK1-PRKN-dependent mitophagy. Authors - Hongxu Xian KD Validated View Article
mouse	WB,IF	J Cell Biol The i-AAA protease YME1L and OMA1 cleave OPA1 to balance mitochondrial fusion and fission. Authors - Ruchika Anand View Article
human	WB	EMBO J FUNDC1 regulates mitochondrial dynamics at the ER-mitochondrial contact site under hypoxic conditions. Authors - Wenxian Wu KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 16413-1-AP

WB analysis of HEK-293T using 16413-1-AP

WB analysis of HT-1080 using 16413-1-AP

WB analysis of HT-1080 using 16413-1-AP

WB analysis of HT-1080 using 16413-1-AP

IHC staining of human liver cancer using 16413-1-AP

IHC staining of human liver cancer using 16413-1-AP

IHC staining of human skeletal muscle using 16413-1-AP

IHC staining of human skeletal muscle using 16413-1-AP

SMCR7/MID49 Polyclonal antibody

SMCR7/MID49 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division.

Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.

MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.

Loss of MIEF1/MiD51 confers susceptibility to BAX-mediated cell death and PINK1-PRKN-dependent mitophagy.

The i-AAA protease YME1L and OMA1 cleave OPA1 to balance mitochondrial fusion and fission.

FUNDC1 regulates mitochondrial dynamics at the ER-mitochondrial contact site under hypoxic conditions.