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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SMN-Exon7 Monoclonal antibody, PBS Only

SMN-Exon7 Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3A8G11

Cat no : 60255-1-PBS

Synonyms

Component of gems 1, Gemin 1, SMN, SMN1, SMN2, SMNC, SMN-Exon7, SMNT, Survival motor neuron protein, T BCD541

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using SMN-Exon7 Monoclonal antibody (60255-1-Ig)

    WB analysis of HEK-293 using 60255-1-Ig (same clone as 60255-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 60255-1-Ig (SMN-Exon7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60255-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using SMN-Exon7 Monoclonal antibody (60255-1-Ig)

    WB analysis of HepG2 using 60255-1-Ig (same clone as 60255-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 60255-1-Ig (SMN-Exon7 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60255-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using SMN-Exon7 Monoclonal antibody (60255-1-Ig)

    WB analysis of HeLa using 60255-1-Ig (same clone as 60255-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 60255-1-Ig (SMN-Exon7 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60255-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SMN-Exon7 Monoclonal antibody (60255-1-Ig)

    IF Staining of HepG2 using 60255-1-Ig (same clone as 60255-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 60255-1-Ig (SMN-Exon7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 60255-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60255-1-PBS targets SMN-Exon7 in WB, IF, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SMN-Exon7 fusion protein Ag16615 相同性解析による交差性が予測される生物種
Full Name survival of motor neuron 1, telomeric
Calculated molecular weight 294 aa, 32 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC062723
Gene symbol SMN1
Gene ID (NCBI) 6606
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Spinal muscular atrophy (SMA) is an autosomal recessive neurodegenerative disease characterized by loss of anterior horn cells in the spinal cord and concomitant symmetrical muscle weakness and atrophy (PMID: 16364894 ). SMA is caused by deletion or mutations of the survival motor neuron (SMN1) gene. SMA patients lack a functional SMN1 gene, but they possess an intact SMN2 gene, which though nearly identical to SMN1, is only partially functional (PMID: 17355180). A large majority of SMN2 transcripts lack exon 7, resulting in production of a truncated, less stable SMN protein (PMID: 10369862). The level of SMN protein correlates with phenotypic severity of SMA. This antibody, 60255-1-Ig, raised against the C-terminal region (275-294aa) encoded by the exon 7.