SMN (Human,Mouse,Rat) Monoclonal antibody, PBS Only

WB result of SMN (Human,Mouse,Rat) antibody (60154-2-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMN (Human,Mouse,Rat) transfected HeLa cells. This data was developed using the same antibody clone with 60154-2-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60154-2-PBS in a different storage buffer formulation.

A375 cells were subjected to SDS PAGE followed by western blot with 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60154-2-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60154-2-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60154-2-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60154-2-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60154-2-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60154-2-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60154-2-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60154-2-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60154-2-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60154-2-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60154-2-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60154-2-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60154-2-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60154-2-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60154-2-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMN (Human,Mouse,Rat) antibody (60154-2-Ig, Clone: 3C10B5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 60154-2-PBS in a different storage buffer formulation.

1x10^6 Jurkat cells were intracellularly stained with 0.25 ug SMN (Human,Mouse,Rat) Monoclonal antibody (60154-2-Ig, Clone:3C10B5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60154-2-PBS in a different storage buffer formulation.