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  • Featured Product
  • KD/KO Validated

SMN (Human-Specific) Monoclonal antibody, PBS Only

SMN (Human-Specific) Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C6D9

Cat no : 60154-1-PBS

Synonyms

SMN, Gemin-1, Gemin 1, Component of gems 1, C BCD541

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SMN (Human-Specific) Monoclonal antibody (60154-1-Ig)

    WB analysis using 60154-1-Ig (same clone as 60154-1-PBS)

    A375, HEK-293, and Jurkat cells were subjected to SDS PAGE followed by western blot with 60154-1-Ig (SMN (Human-Specific) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60154-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using SMN (Human-Specific) Monoclonal antibody (60154-1-Ig)

    WB analysis of HEK-293 using 60154-1-Ig (same clone as 60154-1-PBS)

    WB result of SMN (Human-Specific) antibody (60154-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMN (Human-Specific) transfected HEK-293 cells. This data was developed using the same antibody clone with 60154-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using SMN (Human-Specific) Monoclonal antibody (60154-1-Ig)

    WB analysis of HepG2 using 60154-1-Ig (same clone as 60154-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 60154-1-Ig(SMN (Human-Specific) antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60154-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using SMN (Human-Specific) Monoclonal antibody (60154-1-Ig)

    WB analysis of Raji using 60154-1-Ig (same clone as 60154-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 60154-1-Ig(SMN (Human-Specific) antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60154-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using SMN (Human-Specific) Monoclonal antibody (60154-1-Ig)

    IP experiment of HEK-293 using 60154-1-Ig (same clone as 60154-1-PBS)

    IP result of anti-SMN (Human-Specific) (IP:60154-1-Ig, 4ug; Detection:60154-1-Ig 1:500) with HEK-293 cells lysate 2440ug. This data was developed using the same antibody clone with 60154-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using SMN (Human-Specific) Monoclonal antibody (60154-1-Ig)

    IHC staining of human brain using 60154-1-Ig (same clone as 60154-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 60154-1-Ig(SMN (Human-Specific) antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60154-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer using SMN (Human-Specific) Monoclonal antibody (60154-1-Ig)

    IHC staining of human colon cancer using 60154-1-Ig (same clone as 60154-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 60154-1-Ig (SMN (Human-Specific) antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60154-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer using SMN (Human-Specific) Monoclonal antibody (60154-1-Ig)

    IHC staining of human colon cancer using 60154-1-Ig (same clone as 60154-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 60154-1-Ig (SMN (Human-Specific) antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60154-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SMN (Human-Specific) Monoclonal antibody (60154-1-Ig)

    IHC staining of human kidney using 60154-1-Ig (same clone as 60154-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 60154-1-Ig(SMN (Human-Specific) antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60154-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SMN (Human-Specific) Monoclonal antibody (60154-1-Ig)

    IHC staining of human kidney using 60154-1-Ig (same clone as 60154-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 60154-1-Ig(SMN (Human-Specific) antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60154-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using SMN (Human-Specific) Monoclonal antibody (60154-1-Ig)

    IHC staining of human liver using 60154-1-Ig (same clone as 60154-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 60154-1-Ig(SMN (Human-Specific) antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60154-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using SMN (Human-Specific) Monoclonal antibody (60154-1-Ig)

    IHC staining of human liver using 60154-1-Ig (same clone as 60154-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 60154-1-Ig(SMN (Human-Specific) antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60154-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using SMN (Human-Specific) Monoclonal antibody (60154-1-Ig)

    IHC staining of human heart using 60154-1-Ig (same clone as 60154-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 60154-1-Ig(SMN (Human-Specific) antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60154-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using SMN (Human-Specific) Monoclonal antibody (60154-1-Ig)

    IHC staining of human heart using 60154-1-Ig (same clone as 60154-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 60154-1-Ig(SMN (Human-Specific) antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60154-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SMN (Human-Specific) Monoclonal antibody (60154-1-Ig)

    IF Staining of HepG2 using 60154-1-Ig (same clone as 60154-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMN (Human-Specific) antibody (60154-1-Ig, Clone: 2C6D9 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 60154-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Jurkat cells using SMN (Human-Specific) Monoclonal antibody (60154-1-Ig)

    FC experiment of Jurkat using 60154-1-Ig (same clone as 60154-1-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.4 ug SMN (Human-Specific) Monoclonal antibody (60154-1-Ig, Clone: 2C6D9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60154-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60154-1-PBS targets SMN (Human-Specific) in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SMN (Human-Specific) fusion protein Ag14333 相同性解析による交差性が予測される生物種
Full Name survival of motor neuron 2, centromeric
Calculated molecular weight 282 aa, 30 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC000908
Gene symbol SMN
Gene ID (NCBI) 6607
RRIDAB_10639513
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The survival of motor neurons (SMN) genes are the disease genes of spinal muscular atrophy (SMA), a common motor neuron degenerative disease. The level of SMN protein correlates with phenotypic severity of SMA. SMA patients lack a functional SMN1 gene, but they possess an intact SMN2 gene, which though nearly identical to SMN1, is only partially functional, because a large majority of SMN2 transcripts lack exon 7, resulting in production of a truncated, less stable SMN protein. This antibody 60154-1-Ig is specific to human SMN2. It can't recognize mouse and rat SMN.