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SMN Polyclonal antibody

SMN Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 22329-1-AP

Synonyms

SMN1,SMN, SMN1, Gemin-1, Gemin 1, Component of gems 1


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Tested Applications

Positive WB detected inK-562 cells, HepG2 cells, HEK-293 cells, Jurkat cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22329-1-AP targets SMN in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SMN fusion protein Ag17798 相同性解析による交差性が予測される生物種
Full Name survival of motor neuron 2, centromeric
Calculated molecular weight 282 aa, 30 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC000908
Gene symbol SMN
Gene ID (NCBI) 6607
RRIDAB_2879074
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Spinal muscular atrophy (SMA) is an autosomal recessive neurodegenerative disease characterized by loss of anterior horn cells in the spinal cord and concomitant symmetrical muscle weakness and atrophy (PMID: 16364894 ). SMA is caused by deletion or mutations of the survival motor neuron (SMN1) gene. SMA patients lack a functional SMN1 gene, but they possess an intact SMN2 gene, which though nearly identical to SMN1, is only partially functional (PMID: 17355180). A large majority of SMN2 transcripts lack exon 7, resulting in production of a truncated, less stable SMN protein (PMID: 10369862). The level of SMN protein correlates with phenotypic severity of SMA.

Protocols

Product Specific Protocols
WB protocol for SMN antibody 22329-1-APDownload protocol
IHC protocol for SMN antibody 22329-1-APDownload protocol
IF protocol for SMN antibody 22329-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols