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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SMNDC1 Monoclonal antibody (68344-1-Ig)

    WB analysis using 68344-1-Ig (same clone as 68344-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68344-1-Ig (SMNDC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68344-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using SMNDC1 Monoclonal antibody (68344-1-Ig)

    FC experiment of HeLa using 68344-1-Ig (same clone as 68344-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug SMNDC1 Monoclonal antibody (68344-1-Ig, Clone: 1B10C2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1) (red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 68344-1-PBS in a different storage buffer formulation.

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SMNDC1 Monoclonal antibody, PBS Only

SMNDC1 Monoclonal Antibody for Indirect ELISA, WB
Cat No. 68344-1-PBS
Clone No.1B10C2

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Indirect ELISA, WB

Synonyms

Survival motor neuron domain-containing protein 1, SPF30, SMN-related protein, SMNR, SMN related protein

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68344-1-PBS targets SMNDC1 in Indirect ELISA, WB applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SMNDC1 fusion protein Ag33140 相同性解析による交差性が予測される生物種
Full Name survival motor neuron domain containing 1
Calculated molecular weight 238 aa, 27 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC011234
Gene Symbol SMNDC1
Gene ID (NCBI) 10285
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO75940
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Survival motor neuron domain-containing protein 1 (SMNDC1), also known as survival of motor neuron-related-splicing factor 30 (SPF30) or SMN-related protein (SMNR), is a constituent of the spliceosome complex that plays an essential role in spliceosome assembly by recruiting U4/U5/U6 tri-snRNP to the pre-spliceosomal complex (PMID: 9817934; 11331595; 30904945). Both SMNDC1 and SMN1 contain a highly conserved central Tudor domain which functions as a protein-protein interaction surface and mediates binding to the spliceosomal Sm proteins (PMID: 11331595).