SMO Polyclonal antibody
SMO Polyclonal Antibody for IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 20787-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive IHC detected in | human prostate cancer tissue, human testis tissue, human colon cancer tissue, mouse testis tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
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Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
IHC | See 5 publications below |
IF | See 6 publications below |
Product Information
20787-1-AP targets SMO in IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, chicken |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | SMO fusion protein Ag14322 相同性解析による交差性が予測される生物種 |
Full Name | smoothened homolog (Drosophila) |
Calculated molecular weight | 787 aa, 86 kDa |
GenBank accession number | BC009989 |
Gene symbol | SMO |
Gene ID (NCBI) | 6608 |
RRID | AB_2878740 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Smoothened (SMO) is a G protein-coupled receptor that is a component of the hedgehog (Hh) signaling pathway and is conserved from flies to humans. The Hh pathway plays a central role in animal development and stem-cell function. Defects in Hh signaling lead to birth defects and cancer in humans. Expressed on the primary cilium, SMO interacts with the patched protein, a receptor for Hh proteins.
Protocols
Product Specific Protocols | |
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IHC protocol for SMO antibody 20787-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mol Cancer Circular RNA circIPO11 drives self-renewal of liver cancer initiating cells via Hedgehog signaling. | ||
Sci Adv Primary cilium remodeling mediates a cell signaling switch in differentiating neurons. | ||
Nat Commun TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling. | ||
J Cell Biol Sterol and oxysterol synthases near the ciliary base activate the Hedgehog pathway. | ||
Acta Neuropathol Commun Meningioma cells express primary cilia but do not transduce ciliary Hedgehog signals. | ||
Cell Death Discov N6-methyladenine-mediated aberrant activation of the lncRNA SOX2OT-GLI1 loop promotes non-small-cell lung cancer stemness |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Boyan (Verified Customer) (05-14-2019) | For IF, it could label the ciliary localisation of endogenous Smo; for WB, it could label many bands around 100 kd.
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