SMO Polyclonal antibody

SMO Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20787-1-AP

Synonyms

Gx, Protein Gx, SMO, SMOH, Smoothened homolog


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Tested Applications

Positive IHC detected inhuman prostate cancer tissue, human testis tissue, human colon cancer tissue, mouse testis tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20787-1-AP targets SMO in IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SMO fusion protein Ag14322 相同性解析による交差性が予測される生物種
Full Name smoothened homolog (Drosophila)
Calculated molecular weight 787 aa, 86 kDa
GenBank accession numberBC009989
Gene symbol SMO
Gene ID (NCBI) 6608
RRIDAB_2878740
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Smoothened (SMO) is a G protein-coupled receptor that is a component of the hedgehog (Hh) signaling pathway and is conserved from flies to humans. The Hh pathway plays a central role in animal development and stem-cell function. Defects in Hh signaling lead to birth defects and cancer in humans. Expressed on the primary cilium, SMO interacts with the patched protein, a receptor for Hh proteins.

Protocols

Product Specific Protocols
IHC protocol for SMO antibody 20787-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouse

Mol Cancer

Circular RNA circIPO11 drives self-renewal of liver cancer initiating cells via Hedgehog signaling.

Authors - Yang Gu
chickenIF

Sci Adv

Primary cilium remodeling mediates a cell signaling switch in differentiating neurons.

Authors - Gabriela Toro-Tapia
humanIHC

Nat Commun

TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling.

Authors - Rongxuan Zhu
mouseIF

J Cell Biol

Sterol and oxysterol synthases near the ciliary base activate the Hedgehog pathway.

Authors - Sarah Findakly
humanIF

Acta Neuropathol Commun

Meningioma cells express primary cilia but do not transduce ciliary Hedgehog signals.

Authors - Sarah Findakly
human

Cell Death Discov

N6-methyladenine-mediated aberrant activation of the lncRNA SOX2OT-GLI1 loop promotes non-small-cell lung cancer stemness

Authors - Hongliang Dong

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Boyan (Verified Customer) (05-14-2019)

For IF, it could label the ciliary localisation of endogenous Smo; for WB, it could label many bands around 100 kd.

  • Applications: Western Blot, Immunofluorescence,
  • Cell Tissue Type: MEF