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SMO Monoclonal antibody

SMO Monoclonal Antibody for WB, IF, IHC, ELISA
Cat No. 66851-1-Ig
Clone No.2B10A11

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Canine, Mouse, Rat and More (3)

Applications

WB, IHC, IF/ICC, ELISA

Gx, Protein Gx, SMO, SMOH, Smoothened homolog

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -


ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。


国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inrat brain tissue, mouse brain tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMDCK cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66851-1-Ig targets SMO in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Canine, Mouse, Rat samples.

Tested Reactivity Human, Canine, Mouse, Rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SMO fusion protein Ag25861 相同性解析による交差性が予測される生物種
Full Name smoothened homolog (Drosophila)
Calculated molecular weight 787 aa, 86 kDa
Observed molecular weight86 kDa
GenBank accession numberBC009989
Gene Symbol SMO
Gene ID (NCBI) 6608
RRIDAB_2882191
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99835
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Smoothened (SMO) is a G protein-coupled receptor that is a component of the hedgehog (Hh) signaling pathway and is conserved from flies to humans. The Hh pathway plays central roles in animal development and stem-cell function. Defects in Hh signalling lead to birth defects and cancer in humans. Expressed on the primary cilium, SMO interacts with the patched protein, a receptor for Hh proteins.

Protocols

Product Specific Protocols
WB protocol for SMO antibody 66851-1-IgDownload protocol
IHC protocol for SMO antibody 66851-1-IgDownload protocol
IF protocol for SMO antibody 66851-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC,IF

Int J Biol Sci

Drug Repurposing Flubendazole to Suppress Tumorigenicity via PCSK9-dependent Inhibition and Potentiate Lenvatinib Therapy for Hepatocellular Carcinoma

Authors - Wenjiao Jin
human,mouseWB

iScience

Mutational burden of XPNPEP3 leads to defects in mitochondrial complex I and cilia in NPHPL1

Authors - Lingxiao Tong
humanWB

Front Cell Dev Biol

Microtubule Stabilization Enhances the Chondrogenesis of Synovial Mesenchymal Stem Cells.

Authors - Jiawei Li
ratWB

Oxid Med Cell Longev

Mitochondrial Protection and Against Glutamate Neurotoxicity via Shh/Ptch1 Signaling Pathway to Ameliorate Cognitive Dysfunction by Kaixin San in Multi-Infarct Dementia Rats

Authors - Xiaoqiong Li
humanWB

Cancer Sci

Transcription factor POU4F2 promotes colorectal cancer cell migration and invasion through hedgehog-mediated epithelial-mesenchymal transition.

Authors - Kaibo Guo
humanWB

FASEB J

Inhibition of CK2α accelerates skin wound healing by promoting endothelial cell proliferation through the Hedgehog signaling pathway

Authors - Junjie Zhu
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