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  • KD/KO Validated

SMPD1,ASM Polyclonal antibody

SMPD1,ASM Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14609-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

ASM, SMPD1, SMPD1,ASM

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inmouse brain tissue, mouse cerebellum tissue
Positive IHC detected inhuman brain tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14609-1-AP targets SMPD1,ASM in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SMPD1,ASM fusion protein Ag6180 相同性解析による交差性が予測される生物種
Full Name sphingomyelin phosphodiesterase 1, acid lysosomal
Calculated molecular weight 70 kDa
Observed molecular weight60 kDa, 70-75 kDa
GenBank accession numberBC041164
Gene Symbol SMPD1
Gene ID (NCBI) 6609
RRIDAB_2878068
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP17405
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SMPD1(Sphingomyelin phosphodiesterase) is also named as ASM and belongs to the acid sphingomyelinase family. It is an important enzyme in sphingolipid metabolism and plays key roles in apoptosis, immunity, development, and cancer. The deficient activity of this enzyme results in Types A and B Niemann-Pick disease (NPD). This protein has 4 isoforms produced by alternative splicing. SMPD1 can produce a 60 kDa peptide with its signal peptide missing.

Protocols

Product Specific Protocols
WB protocol for SMPD1,ASM antibody 14609-1-APDownload protocol
IHC protocol for SMPD1,ASM antibody 14609-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse

Cell Host Microbe

The β2Integrin Mac-1 Induces Protective LC3-Associated Phagocytosis of Listeria monocytogenes.

Authors - Alexander Gluschko
  • KO Validated
humanWB,IF

Mol Ther

Polyphyllin D "Punctures" Hypertrophic Lysosomes to Reverse Drug Resistance of Hepatocellular Carcinoma by Targeting Acid Sphingomyelinase

Authors - Yang Wang
human,mouseWB

Cancer Res

Aneuploid cell survival relies upon sphingolipid homeostasis.

Authors - Yun-Chi Tang
mouseIF

Acta Neuropathol Commun

Inhibition of acid sphingomyelinase reduces reactive astrocyte secretion of mitotoxic extracellular vesicles and improves Alzheimer's disease pathology in the 5xFAD mouse

Authors - Simone M Crivelli
mouseIF

Cells

Novel Isolation Method Reveals Sex-Specific Composition and Neurotoxicity of Small Extracellular Vesicles in a Mouse Model of Alzheimer's Disease

Authors - Ahmed Elsherbini
humanIHC

J Mol Med (Berl)

Acid sphingomyelinase expression is associated with survival in resectable pancreatic ductal adenocarcinoma

Authors - Gregory C Wilson
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