Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse brain tissue, mouse cerebellum tissue |
| Positive IHC detected in | human brain tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 10 publications below |
| IHC | See 3 publications below |
| IF | See 6 publications below |
Product Information
14609-1-AP targets SMPD1,ASM in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SMPD1,ASM fusion protein Ag6180 相同性解析による交差性が予測される生物種 |
| Full Name | sphingomyelin phosphodiesterase 1, acid lysosomal |
| Calculated molecular weight | 70 kDa |
| Observed molecular weight | 60 kDa, 70-75 kDa |
| GenBank accession number | BC041164 |
| Gene Symbol | SMPD1 |
| Gene ID (NCBI) | 6609 |
| RRID | AB_2878068 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P17405 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SMPD1(Sphingomyelin phosphodiesterase) is also named as ASM and belongs to the acid sphingomyelinase family. It is an important enzyme in sphingolipid metabolism and plays key roles in apoptosis, immunity, development, and cancer. The deficient activity of this enzyme results in Types A and B Niemann-Pick disease (NPD). This protein has 4 isoforms produced by alternative splicing. SMPD1 can produce a 60 kDa peptide with its signal peptide missing.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SMPD1,ASM antibody 14609-1-AP | Download protocol |
| IHC protocol for SMPD1,ASM antibody 14609-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Host Microbe The β2Integrin Mac-1 Induces Protective LC3-Associated Phagocytosis of Listeria monocytogenes.
| ||
Mol Ther Polyphyllin D "Punctures" Hypertrophic Lysosomes to Reverse Drug Resistance of Hepatocellular Carcinoma by Targeting Acid Sphingomyelinase | ||
Acta Neuropathol Commun Inhibition of acid sphingomyelinase reduces reactive astrocyte secretion of mitotoxic extracellular vesicles and improves Alzheimer's disease pathology in the 5xFAD mouse | ||
Cells Novel Isolation Method Reveals Sex-Specific Composition and Neurotoxicity of Small Extracellular Vesicles in a Mouse Model of Alzheimer's Disease | ||
J Mol Med (Berl) Acid sphingomyelinase expression is associated with survival in resectable pancreatic ductal adenocarcinoma |