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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SMS Monoclonal antibody (68040-1-Ig)

    WB analysis using 68040-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 68040-1-Ig (SMS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of K-562 cells using SMS Monoclonal antibody (68040-1-Ig)

    WB analysis of K-562 using 68040-1-Ig

    WB result of SMS antibody (68040-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMS transfected K-562 cells.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SMS Monoclonal antibody (68040-1-Ig)

    IF Staining of HeLa using 68040-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SMS antibody (68040-1-Ig, Clone: 1G8E9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using SMS Monoclonal antibody (68040-1-Ig)

    FC experiment of HeLa using 68040-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SMS (68040-1-Ig, Clone:1G8E9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  • Featured Product
  • KD/KO Validated

SMS Monoclonal antibody

SMS Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 68040-1-Ig
Clone No.1G8E9

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

Spermine synthase, 1G8E9, EC:2.5.1.22, MRSR, SPMSY

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, K-562 cells, HCT 116 cells, HEK-293 cells, Hela cells, HepG2 cells, Jurkat cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68040-1-Ig targets SMS in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SMS fusion protein Ag8840 相同性解析による交差性が予測される生物種
Full Name spermine synthase
Calculated molecular weight 366 aa, 41 kDa
Observed molecular weight 41 kDa
GenBank accession numberBC009898
Gene Symbol SMS
Gene ID (NCBI) 6611
RRIDAB_2918782
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP52788
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SMS antibody 68040-1-IgDownload protocol
IF protocol for SMS antibody 68040-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols