Validation Data Gallery

Filter: AllWBIHC

WB analysis of COLO 320 using 23567-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 23567-1-AP (SMTN Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 23567-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 23567-1-AP (SMTN Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.
IHC staining of human hysteromyoma using 23567-1-AP
Immunohistochemical analysis of paraffin-embedded human hysteromyoma tissue slide using 23567-1-AP (SMTN Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human hysteromyoma using 23567-1-AP
Immunohistochemical analysis of paraffin-embedded human hysteromyoma tissue slide using 23567-1-AP (SMTN Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human hysteromyoma using 23567-1-AP
Immunohistochemical analysis of paraffin-embedded human hysteromyoma tissue slide using 23567-1-AP (SMTN Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human hysteromyoma using 23567-1-AP
Immunohistochemical analysis of paraffin-embedded human hysteromyoma tissue slide using 23567-1-AP (SMTN Antibody) at dilution of 1:200 (under 10x lens).

SMTN Polyclonal antibody

SMTN Polyclonal Antibody for WB, IHC, ELISA

Cat No. 23567-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

mouse, human, rat

Applications

WB, IHC, ELISA

Synonyms

smoothelin, SMSMO, SMTN

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (5)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	COLO 320 cells
Positive IHC detected in	human hysteromyoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 5 publications below
IHC	See 2 publications below

Product Information

23567-1-AP targets SMTN in WB, IHC, ELISA applications and shows reactivity with mouse, human, rat samples.

Tested Reactivity	mouse, human, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SMTN fusion protein Ag19983 相同性解析による交差性が予測される生物種
Full Name	smoothelin
Calculated molecular weight	940 aa, 102 kDa
Observed molecular weight	117 kDa
GenBank accession number	BC034237
Gene Symbol	SMTN
Gene ID (NCBI)	6525
RRID	AB_2879298
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen Affinity purified
UNIPROT ID	P53814
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SMTN, also named as Smoothelin, is a 917 amino acid protein, which contains 1 CH (calponin-homology) domain and belongs to the smoothelin family. SMTN as structural protein of the cytoskeleton involve in muscle organ development and smooth muscle contraction.

Protocols

Product Specific Protocols
WB protocol for SMTN antibody 23567-1-AP	Download protocol
IHC protocol for SMTN antibody 23567-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC	bioRxiv Coordination between cytoskeletal organization, cell contraction and extracellular matrix development, is depended on LOX for aneurysm prevention Authors - Rohtem Aviram View Article
human	WB,IHC	FEBS J Coordination among cytoskeletal organization, cell contraction, and extracellular matrix development is dependent on LOX for aneurysm prevention Authors - Rohtem Aviram View Article
mouse	WB	Am J Physiol Renal Physiol PMID: 31066574 Authors - Nao Iguchi View Article
human	WB	Int J Nanomedicine Glycosylation-Engineered Platelet Membrane-Coated Interleukin 10 Nanoparticles for Targeted Inhibition of Vascular Restenosis Authors - Fengshi Li View Article
human,mouse	WB	J Mol Med (Berl) Vav2 promotes ductus arteriosus anatomic closure via the remodeling of smooth muscle cells by Rac1 activation Authors - Yinghui Chen View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of COLO 320 using 23567-1-AP

WB analysis of COLO 320 using 23567-1-AP

IHC staining of human hysteromyoma using 23567-1-AP

IHC staining of human hysteromyoma using 23567-1-AP

IHC staining of human hysteromyoma using 23567-1-AP

IHC staining of human hysteromyoma using 23567-1-AP

SMTN Polyclonal antibody

SMTN Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Coordination between cytoskeletal organization, cell contraction and extracellular matrix development, is depended on LOX for aneurysm prevention

Coordination among cytoskeletal organization, cell contraction, and extracellular matrix development is dependent on LOX for aneurysm prevention

PMID: 31066574

Glycosylation-Engineered Platelet Membrane-Coated Interleukin 10 Nanoparticles for Targeted Inhibition of Vascular Restenosis

Vav2 promotes ductus arteriosus anatomic closure via the remodeling of smooth muscle cells by Rac1 activation