• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SMTNL2 Polyclonal antibody

SMTNL2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14782-1-AP

Synonyms

Smoothelin-like protein 2, Smoothelin like protein 2, smoothelin like 2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of COLO 320 cells using SMTNL2 Polyclonal antibody (14782-1-AP)

    WB analysis of COLO 320 using 14782-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 14782-1-AP (SMTNL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse small intestine tissue using SMTNL2 Polyclonal antibody (14782-1-AP)

    WB analysis of mouse small intestine using 14782-1-AP

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 14782-1-AP (SMTNL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using SMTNL2 Polyclonal antibody (14782-1-AP)

    WB analysis of MCF-7 using 14782-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 14782-1-AP (SMTNL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SGC-7901 cells using SMTNL2 Polyclonal antibody (14782-1-AP)

    WB analysis of SGC-7901 using 14782-1-AP

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 14782-1-AP (SMTNL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of COLO 320 cells using SMTNL2 Polyclonal antibody (14782-1-AP)

    IP experiment of COLO 320 using 14782-1-AP

    IP result of anti-SMTNL2 (IP:14782-1-AP, 4ug; Detection:14782-1-AP 1:500) with COLO 320 cells lysate 4000ug.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using SMTNL2 Polyclonal antibody (14782-1-AP)

    IF Staining of MCF-7 using 14782-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 14782-1-AP (SMTNL2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Positive WB detected inCOLO 320 cells, MCF-7 cells, mouse small intestine tissue, SGC-7901 cells
Positive IP detected inCOLO 320 cells
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14782-1-AP targets SMTNL2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SMTNL2 fusion protein Ag6395 相同性解析による交差性が予測される生物種
Full Name smoothelin-like 2
Calculated molecular weight 50 kDa
Observed molecular weight 68-70 kDa
GenBank accession numberBC112177
Gene symbol SMTNL2
Gene ID (NCBI) 342527
RRIDAB_2270842
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SMTNL2 antibody 14782-1-APDownload protocol
IF protocol for SMTNL2 antibody 14782-1-APDownload protocol
IP protocol for SMTNL2 antibody 14782-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols