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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SMU1 Polyclonal antibody

SMU1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15511-1-AP

Synonyms

WD40 repeat-containing protein SMU1, Smu-1 suppressor of mec-8 and unc-52 protein homolog, SMU 1, SAP57, fSAP57

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using SMU1 Polyclonal antibody (15511-1-AP)

    WB analysis of mouse liver using 15511-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 15511-1-AP (SMU1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using SMU1 Polyclonal antibody (15511-1-AP)

    WB analysis of K-562 using 15511-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 15511-1-AP (SMU1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SMU1 Polyclonal antibody (15511-1-AP)

    IHC staining of human kidney using 15511-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15511-1-AP (SMU1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using SMU1 Polyclonal antibody (15511-1-AP)

    IHC staining of human kidney using 15511-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15511-1-AP (SMU1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using SMU1 Polyclonal antibody (15511-1-AP)

    IHC staining of human testis using 15511-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 15511-1-AP (SMU1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using SMU1 Polyclonal antibody (15511-1-AP)

    IHC staining of human testis using 15511-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 15511-1-AP (SMU1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using SMU1 Polyclonal antibody (15511-1-AP)

    IHC staining of human brain using 15511-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15511-1-AP (SMU1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using SMU1 Polyclonal antibody (15511-1-AP)

    IHC staining of human brain using 15511-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15511-1-AP (SMU1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using SMU1 Polyclonal antibody (15511-1-AP)

    IHC staining of human ovary using 15511-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 15511-1-AP (SMU1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using SMU1 Polyclonal antibody (15511-1-AP)

    IHC staining of human ovary using 15511-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 15511-1-AP (SMU1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using SMU1 Polyclonal antibody (15511-1-AP)

    IHC staining of human kidney using 15511-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15511-1-AP (SMU1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using SMU1 Polyclonal antibody (15511-1-AP)

    IHC staining of human kidney using 15511-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15511-1-AP (SMU1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using SMU1 Polyclonal antibody (15511-1-AP)

    IHC staining of human hepatocirrhosis using 15511-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 15511-1-AP (SMU1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using SMU1 Polyclonal antibody (15511-1-AP)

    IHC staining of human hepatocirrhosis using 15511-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 15511-1-AP (SMU1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SMU1 Polyclonal antibody (15511-1-AP)

    IF Staining of HepG2 using 15511-1-AP

    Immunofluorescent analysis of HepG2 cells, using SMU1 antibody 15511-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inmouse liver tissue, K-562 cells
Positive IHC detected inhuman kidney tissue, human brain tissue, human hepatocirrhosis tissue, human ovary tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15511-1-AP targets SMU1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SMU1 fusion protein Ag7688 相同性解析による交差性が予測される生物種
Full Name smu-1 suppressor of mec-8 and unc-52 homolog (C. elegans)
Calculated molecular weight 58 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC002876
Gene symbol SMU1
Gene ID (NCBI) 55234
RRIDAB_2270843
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sum1, belongs to the WD repeat SMU1 family. Human SMU1 is a component of the spliceosome fSAP57(PMID: 15254247). It is possible that the mutation in Smu1 affects spliceosome function, resulting in generation of improper splice variants, which could explain the phenotypes of tsTM18 cells. Some results suggest that Smu1 participates in at least two steps of splicing: splice-site choice and control of accuracy(PMID: 17105761). This antibody is a rabbit polyclonal antibody raised against a region of human REDD1 antigen.

Protocols

Product Specific Protocols
WB protocol for SMU1 antibody 15511-1-APDownload protocol
IHC protocol for SMU1 antibody 15511-1-APDownload protocol
IF protocol for SMU1 antibody 15511-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols