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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SMUG1 Recombinant antibody (83771-5-RR)

    WB analysis using 83771-5-RR (same clone as 83771-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83771-5-RR (SMUG1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83771-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U-2 OS using SMUG1 Recombinant antibody (83771-5-RR)

    FC experiment of U-2 OS using 83771-5-RR (same clone as 83771-5-PBS)

    1x10^6 U-2 OS were intracellularly stained with 0.25 ug SMUG1 Recombinant antibody (83771-5-RR, Clone:240817E4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83771-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83771-5-RR

    Affinity and Kinetic Characterization of 83771-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83771-5-RR against Human SMUG1 were performed. The affinity constant is below 1 pM.

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SMUG1 Recombinant antibody, PBS Only

SMUG1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83771-5-PBS
Clone No.240817E4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

UNG3, Single-strand selective monofunctional uracil DNA glycosylase, HMUDG, FDG, EC:3.2.2.-

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83771-5-PBS targets SMUG1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SMUG1 fusion protein Ag13934 相同性解析による交差性が予測される生物種
Full Name single-strand-selective monofunctional uracil-DNA glycosylase 1
Calculated molecular weight 177 aa, 20 kDa
Observed molecular weight30 kDa
GenBank accession numberBC000417
Gene Symbol SMUG1
Gene ID (NCBI) 23583
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ53HV7
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

UNG(Uracil-DNA glycosylase) removes uracil in DNA resulting from deamination of cytosine or replicative incorporation of dUMP instead of dTMP. Thus, UNG plays a role in suppressing GC-to-AT transition mutations.The UNG gene encodes 2 isoforms that are individually targeted to the mitochondria and the nucleus(PMID:12369930).Defects in UNG are a cause of immunodeficiency with hyper-IgM type 5 (HIGM5).