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KD/KO Validated

SMURF2 Polyclonal antibody

Name: SMURF2 Polyclonal antibody
Brand: Proteintech
SKU: 18038-1-AP

SMURF2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 18038-1-AP

Synonyms

hSMURF2, SMURF2

Validation Data Gallery

Filter:

AllWBIPIHC

WB analysis using 18038-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18038-1-AP (SMURF2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis using 18038-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18038-1-AP (SMURF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse testis using 18038-1-AP
IP result of anti-SMURF2 (IP:18038-1-AP, 4ug; Detection:18038-1-AP 1:300) with mouse testis tissue lysate 4000 ug.
IHC staining of human lung cancer using 18038-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18038-1-AP (SMURF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human malignant melanoma using 18038-1-AP
Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 18038-1-AP (SMURF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 18038-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18038-1-AP (SMURF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat kidney using 18038-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 18038-1-AP (SMURF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat testis using 18038-1-AP
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 18038-1-AP (SMURF2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat testis using 18038-1-AP
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 18038-1-AP (SMURF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 18038-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse testis tissue, rat testis tissue
Positive IHC detected in	human lung cancer tissue, human malignant melanoma tissue, mouse testis tissue, rat kidney tissue, rat testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 1 publications below
IP	See 1 publications below

Product Information

18038-1-AP targets SMURF2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SMURF2 fusion protein Ag12666 相同性解析による交差性が予測される生物種
Full Name	SMAD specific E3 ubiquitin protein ligase 2
Calculated molecular weight	748 aa, 86 kDa
Observed molecular weight	86-100 kDa
GenBank accession number	BC093876
Gene symbol	SMURF2
Gene ID (NCBI)	64750
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SMURF2 is a HECT domain E3 ubiquitin ligase involved in degradation of SMADs,TGF-beta receptor,and other substrates.It also functions in regulation of neuronal and planar cell polarity, induction of senescence, and tumor suppression(PMID:22231558).SMURF2 associates constitutively with SMAD7 and it is nuclear, but binding to SMAD7 induces export and recruitment to activated TGFBR, where it causes degradation of receptors and of SMAD7 via proteasomal and lysosomal pathways(PMIF:11163210). SMURF2 can be detected 100 kDa by poly-sumoylated (PMID:26679521).

Protocols

Product Specific Protocols
WB protocol for SMURF2 antibody 18038-1-AP	Download protocol
IHC protocol for SMURF2 antibody 18038-1-AP	Download protocol
IP protocol for SMURF2 antibody 18038-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Mol Cell SMURF2 predisposes cancer cell toward ferroptosis in GPX4-independent manners by promoting GSTP1 degradation Authors - Wenxin Zhang KD Validated View Article
mouse	IP	Mol Med Acid ceramidase targeting pyruvate kinase affected trypsinogen activation in acute pancreatitis Authors - Juan Xiao View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SMURF2 Polyclonal antibody

SMURF2 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis using 18038-1-AP

WB analysis using 18038-1-AP

IP experiment of mouse testis using 18038-1-AP

IHC staining of human lung cancer using 18038-1-AP

IHC staining of human malignant melanoma using 18038-1-AP

IHC staining of mouse testis using 18038-1-AP

IHC staining of rat kidney using 18038-1-AP

IHC staining of rat testis using 18038-1-AP