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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SMURF2 Recombinant antibody, PBS Only

SMURF2 Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241486F3

Cat no : 84445-1-PBS

Synonyms

SMAD-specific E3 ubiquitin-protein ligase 2, hSMURF2, HECT-type E3 ubiquitin transferase SMURF2, EC:2.3.2.26, E3 ubiquitin-protein ligase SMURF2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using SMURF2 Recombinant antibody (84445-1-RR)

    IF Staining of U2OS using 84445-1-RR (same clone as 84445-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SMURF2 antibody (84445-1-RR, Clone: 241486F3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84445-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U2OS cells using SMURF2 Recombinant antibody (84445-1-RR)

    FC experiment of U2OS using 84445-1-RR (same clone as 84445-1-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug SMURF2 Recombinant antibody (84445-1-RR, Clone:241486F3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84445-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 84445-1-PBS is SMURF2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SMURF2 fusion protein Ag33419 相同性解析による交差性が予測される生物種
Full Name SMAD specific E3 ubiquitin protein ligase 2
Calculated molecular weight 748 aa, 86 kDa
GenBank accession numberBC093876
Gene symbol SMURF2
Gene ID (NCBI) 64750
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SMURF2 is a HECT domain E3 ubiquitin ligase involved in degradation of SMADs,TGF-beta receptor,and other substrates.It also functions in regulation of neuronal and planar cell polarity, induction of senescence, and tumor suppression(PMID:22231558).SMURF2 associates constitutively with SMAD7 and it is nuclear, but binding to SMAD7 induces export and recruitment to activated TGFBR, where it causes degradation of receptors and of SMAD7 via proteasomal and lysosomal pathways(PMIF:11163210).