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SMYD3 Polyclonal antibody

Name: SMYD3 Polyclonal antibody
Brand: Proteintech
SKU: 12011-1-AP
Rating: 4.00 (1 reviews)

SMYD3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 12011-1-AP

Synonyms

ZMYND1, Zinc finger MYND domain-containing protein 1, SET and MYND domain-containing protein 3, Histone-lysine N-methyltransferase SMYD3, EC:2.1.1.354

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of mouse brain using 12011-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 12011-1-AP (SMYD3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12011-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12011-1-AP (SMYD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 12011-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 12011-1-AP (SMYD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 12011-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 12011-1-AP (SMYD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12011-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12011-1-AP (SMYD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 12011-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 12011-1-AP (SMYD3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 12011-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 12011-1-AP (SMYD3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12011-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12011-1-AP (SMYD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 12011-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 12011-1-AP (SMYD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 12011-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 12011-1-AP (SMYD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of COLO 320 using 12011-1-AP
IP result of anti-SMYD3 (IP:12011-1-AP, 4ug; Detection:12011-1-AP 1:500) with COLO 320 cells lysate 1280ug.
IHC staining of human breast cancer using 12011-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12011-1-AP (SMYD3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 12011-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12011-1-AP (SMYD3 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 12011-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12011-1-AP (SMYD3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 12011-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12011-1-AP (SMYD3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 12011-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12011-1-AP (SMYD3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HEK-293 using 12011-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SMYD3 antibody (12011-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 12011-1-AP

Size/Concentration

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse brain tissue, COLO 320 cells, HeLa cells, HepG2 cells
Positive IP detected in	COLO 320 cells
Positive IHC detected in	human breast cancer tissue, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 6 publications below
IHC	See 1 publications below
ChIP	See 1 publications below

Product Information

12011-1-AP targets SMYD3 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SMYD3 fusion protein Ag2624 相同性解析による交差性が予測される生物種
Full Name	SET and MYND domain containing 3
Calculated molecular weight	428aa,49 kDa; 369aa,42 kDa
Observed molecular weight	42-45 kDa
GenBank accession number	BC031010
Gene symbol	SMYD3
Gene ID (NCBI)	64754
RRID	AB_2193981
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SMYD3,also name as ZMYND1 and ZNFN3A1, belongs to the histone-lysine methyltransferase family.It is a histone methyltransferase that plays an important role in transcriptional regulation in human carcinogenesis. It can specifically methylate histone H3 at lysine 4 and activate the transcription of a set of downstream genes, including several oncogenes (e.g., N-myc, CrkL, Wnt10b, RIZ and hTERT) and genes involved in the control of cell cycle.(PMID: 20957523). It plays an important role in transcriptional activation as a member of an RNA polymerase complex. SMYD3 is frequently overexpressed in different types of cancer cells. It functions as a coactivator of Era and potentiates Era activity in response to ligand. SMYD3 as a new coactivator for ER-mediated transcription, providing a possible link between SMYD3 overexpression and breast cancer. (PMID: 19509295) The common variable number of tandem repeats polymorphism in SMYD3 is a susceptibility factor for some types of human cancer. (PMID:16155568 ) . SMYD3 exists some isoforms with MV 49, 43 and 30 kDa.

Protocols

Product Specific Protocols
WB protocol for SMYD3 antibody 12011-1-AP	Download protocol
IHC protocol for SMYD3 antibody 12011-1-AP	Download protocol
IF protocol for SMYD3 antibody 12011-1-AP	Download protocol
IP protocol for SMYD3 antibody 12011-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat	WB	Acta Pharm Sin B SMYD3-PARP16 axis accelerates unfolded protein response and mediates neointima formation. Authors - Fen Long KD Validated View Article
human	WB	Carcinogenesis miR-124 and miR-203 are epigenetically silenced tumor-suppressive microRNAs in hepatocellular carcinoma. Authors - Furuta Mayuko M View Article
human	WB	Int J Androl Histone methylation is a critical regulator of the abnormal expression of POU5F1 and RASSF1A in testis cancer cell lines. Authors - Lambrot R R View Article
human	WB	Med Oncol PMID: 20957523 Authors - Ren Tian-nian TN KD Validated View Article
rat	WB	Cell Physiol Biochem iTRAQ-Based Proteomic Analysis of Neonatal Kidney from Offspring of Protein Restricted Rats Reveals Abnormalities in Intraflagellar Transport Proteins. Authors - Xiaomei Liu View Article
human	WB,IHC	Oncogenesis SMYD3 promotes endometrial cancer through epigenetic regulation of LIG4/XRCC4/XLF complex in non-homologous end joining repair Authors - Yujia Huang View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Deyong (Verified Customer) (03-11-2022)

It worked well in the HEK293T cells with human SMYD3 overexpression.

Applications: Western Blot
Primary Antibody Dilution: 1/500
Cell Tissue Type: HEK293T cells

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SMYD3 Polyclonal antibody

SMYD3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of mouse brain using 12011-1-AP

WB analysis of HeLa using 12011-1-AP

WB analysis of HepG2 using 12011-1-AP

WB analysis of COLO 320 using 12011-1-AP

WB analysis of HeLa using 12011-1-AP

WB analysis of COLO 320 using 12011-1-AP

WB analysis of COLO 320 using 12011-1-AP

WB analysis of HeLa using 12011-1-AP

WB analysis of mouse brain using 12011-1-AP

WB analysis of mouse brain using 12011-1-AP

IP experiment of COLO 320 using 12011-1-AP

IHC staining of human breast cancer using 12011-1-AP

IHC staining of human colon cancer using 12011-1-AP

IHC staining of human breast cancer using 12011-1-AP

IHC staining of human colon cancer using 12011-1-AP

IHC staining of human colon cancer using 12011-1-AP

IF Staining of HEK-293 using 12011-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

SMYD3-PARP16 axis accelerates unfolded protein response and mediates neointima formation.

miR-124 and miR-203 are epigenetically silenced tumor-suppressive microRNAs in hepatocellular carcinoma.

Histone methylation is a critical regulator of the abnormal expression of POU5F1 and RASSF1A in testis cancer cell lines.

PMID: 20957523

iTRAQ-Based Proteomic Analysis of Neonatal Kidney from Offspring of Protein Restricted Rats Reveals Abnormalities in Intraflagellar Transport Proteins.

SMYD3 promotes endometrial cancer through epigenetic regulation of LIG4/XRCC4/XLF complex in non-homologous end joining repair

Reviews

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