SNAI1 Polyclonal antibody
SNAI1 Polyclonal Antibody for WB, IF, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IP, IF, IHC, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 26183-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, NIH/3T3 cells, PC-3 cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 19 publications below |
| IHC | See 3 publications below |
| IF | See 2 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
26183-1-AP targets SNAI1 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SNAI1 fusion protein Ag24248 相同性解析による交差性が予測される生物種 |
| Full Name | snail homolog 1 (Drosophila) |
| Calculated molecular weight | 264 aa, 29 kDa |
| Observed molecular weight | 29-35 kDa |
| GenBank accession number | BC012910 |
| Gene symbol | SNAI1 |
| Gene ID (NCBI) | 6615 |
| RRID | AB_2880415 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SNAI1, a member of SNAI1 family of protein, participates in the epithelial to mesenchymal transition(EMT) and formation and maintenance of embryonic mesoderm. The snail family share a common structural, that a highly conserved C-terminal region containing a zinc finger transcription factor. SNAI1 interacts with other corepressor, such as Ajuba, PRMT5 and SIN3a or HDAC1 and 2, to repress the target gene. As the phosphorylation modification of SNAI1 protein, the range of molecular weight of SNAI1 is about 25-30 kDa (PMID: 22276203 ). Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SNAI1 antibody 26183-1-AP | Download protocol |
| IF protocol for SNAI1 antibody 26183-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oxid Med Cell Longev XPO1-Mediated EIF1AX Cytoplasmic Relocation Promotes Tumor Migration and Invasion in Endometrial Carcinoma | ||
Aging (Albany NY) Membrane-cytoplasm translocation of annexin A4 is involved in the metastasis of colorectal carcinoma. | ||
Front Cell Dev Biol Targeting MALT1 Suppresses the Malignant Progression of Colorectal Cancer via miR-375/miR-365a-3p/NF-κB Axis. | ||
Oncotarget AEG-1/MTDH-activated autophagy enhances human malignant glioma susceptibility to TGF-β1-triggered epithelial-mesenchymal transition. | ||
J Cell Mol Med Autophagy promotes fibrosis and apoptosis in the peritoneum during long-term peritoneal dialysis. | ||
Cancer Manag Res AOC1 Contributes to Tumor Progression by Promoting the AKT and EMT Pathways in Gastric Cancer. |
