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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using SNAI1 Recombinant antibody (82214-2-RR)

    WB analysis of HeLa using 82214-2-RR

    HeLa cells were subjected to SDS PAGE followed by western blot with 82214-2-RR (SNAI1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 82214-2-RR

    Affinity and Kinetic Characterization of 82214-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82214-2-RR against Human SNAI1 were performed. The affinity constant is below 1 pM.

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SNAI1 Recombinant antibody

SNAI1 Recombinant Antibody for WB, ELISA
Cat No. 82214-2-RR
Clone No.230035C9

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

dJ710H13.1, Protein sna, Protein snail homolog 1, SLUGH2, SNA, SNAH, SNAI1, snail homolog 1 (Drosophila), SNAIL1, Zinc finger protein SNAI1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82214-2-RR targets SNAI1 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SNAI1 fusion protein Ag3723 相同性解析による交差性が予測される生物種
Full Name snail homolog 1 (Drosophila)
Calculated molecular weight 264 aa, 29 kDa
Observed molecular weight35 kDa
GenBank accession numberBC012910
Gene Symbol SNAI1
Gene ID (NCBI) 6615
RRIDAB_3670519
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO95863
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SNAI1, a member of SNAI1 family of protein, participates in the epithelial to mesenchymal transition(EMT) and formation and maintenance of embryonic mesoderm. The snail family share a common structural, that a highly conserved C-terminal region containing a zinc finger transcription factor. SNAI1 interacts with other corepressor, such as Ajuba, PRMT5 and SIN3a or HDAC1 and 2, to repress the target gene. As the phosphorylation modification of SNAI1 protein, the range of molecular weight of SNAI1 is about 25-30 kDa (PMID: 22276203 ). Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs.

Protocols

Product Specific Protocols
WB protocol for SNAI1 antibody 82214-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols