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  • Featured Product
  • KD/KO Validated

SNAI3 Polyclonal antibody

SNAI3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21350-1-AP

Synonyms

Zinc finger protein 293, Zfp293, SNAIL3, snail homolog 3 (Drosophila), SMUC

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using SNAI3 Polyclonal antibody (21350-1-AP)

    WB analysis of MCF-7 using 21350-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 21350-1-AP (SNAI3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of T-47D cells using SNAI3 Polyclonal antibody (21350-1-AP)

    WB analysis of T-47D using 21350-1-AP

    T-47D cells were subjected to SDS PAGE followed by western blot with 21350-1-AP (SNAI3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using SNAI3 Polyclonal antibody (21350-1-AP)

    IHC staining of human skin cancer using 21350-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 21350-1-AP (SNAI3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using SNAI3 Polyclonal antibody (21350-1-AP)

    IHC staining of human skin cancer using 21350-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 21350-1-AP (SNAI3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using SNAI3 Polyclonal antibody (21350-1-AP)

    IF Staining of MCF-7 using 21350-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed MCF-7 cells using 21350-1-AP (SNAI3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inMCF-7 cells, T-47D cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21350-1-AP targets SNAI3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SNAI3 fusion protein Ag15722 相同性解析による交差性が予測される生物種
Full Name snail homolog 3 (Drosophila)
Calculated molecular weight 292 aa, 32 kDa
Observed molecular weight 32-38 kDa
GenBank accession numberBC107058
Gene symbol SNAI3
Gene ID (NCBI) 333929
RRIDAB_2878844
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SNAI3 antibody 21350-1-APDownload protocol
IHC protocol for SNAI3 antibody 21350-1-APDownload protocol
IF protocol for SNAI3 antibody 21350-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Growth Factors

MiR-200c regulates invasion, proliferation and EMT of anaplastic thyroid cancer cells by targeting parathyroid hormone like hormone

Authors - Yan Zhang
View Article
humanWB,IF

Front Oncol

Identifying key transcription factors and immune infiltration in non-small-cell lung cancer using weighted correlation network and Cox regression analyses

Authors - Jingyao Zhang
  • KD Validated
View Article