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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SNAP25 Recombinant antibody, PBS Only

SNAP25 Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240069F12

Cat no : 83259-5-PBS

Synonyms

SNAP 25, SNAP, RIC-4, RIC4, RIC 4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SNAP25 Recombinant antibody (83259-5-RR)

    WB analysis using 83259-5-RR (same clone as 83259-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83259-5-RR (SNAP25 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83259-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using SNAP25 Recombinant antibody (83259-5-RR)

    IF Staining of PC-12 using 83259-5-RR (same clone as 83259-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using SNAP25 antibody (83259-5-RR, Clone: 240069F12 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83259-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using SNAP25 Recombinant antibody (83259-5-RR)

    IF Staining of SH-SY5Y using 83259-5-RR (same clone as 83259-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using SNAP25 antibody (83259-5-RR, Clone: 240069F12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83259-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83259-5-RR

    Affinity and Kinetic Characterization of 83259-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83259-5-RR against Human SNAP25 were performed. The affinity constant is 0.388 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83259-5-PBS is SNAP25 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SNAP25 fusion protein Ag6695 相同性解析による交差性が予測される生物種
Full Name synaptosomal-associated protein, 25kDa
Calculated molecular weight 23 kDa
Observed molecular weight25 kDa
GenBank accession numberBC010647
Gene symbol SNAP25
Gene ID (NCBI) 6616
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The synaptosomal associated protein of 25 kD (SNAP-25) was first identified as a major synaptic protein by Wilson and colleagues. The protein interacts with syntaxin and synaptobrevin through its N-terminal and C-terminal -helical domains. Its palmitoylation domain is located in the middle of the molecule that contains four cysteine residues. Mutation of the cysteines abolishes palmitoylation and membrane binding. Several elegant studies using synaptosome preparations and permeabilized PC12 cells have suggested that SNAP-25 may act in the late post-docking steps of exocytosis. By limited proteolysis and in vitro binding assay, it is proposed that the two helix domains act independently and contribute equally to form the SNARE complex with syntaxin and synaptobrevin. It seems that a major regulatory element is located in the C-terminus of SNAP-25. Removing a 9 amino acid sequence of SNAP-25 inhibited neurosecretion in chromaffin cells.