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SNAP23 Polyclonal antibody
SNAP23 Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF, IP, ELISA
Conjugate
Unconjugated
Cat no : 10825-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human placenta tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 17 publications below |
| IHC | See 3 publications below |
| IF | See 7 publications below |
Product Information
10825-1-AP targets SNAP23 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SNAP23 fusion protein Ag1269 相同性解析による交差性が予測される生物種 |
| Full Name | synaptosomal-associated protein, 23kDa |
| Calculated molecular weight | 23 kDa |
| Observed molecular weight | 23-25 kDa |
| GenBank accession number | BC000148 |
| Gene symbol | SNAP23 |
| Gene ID (NCBI) | 8773 |
| RRID | AB_2192022 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SNAP23 antibody 10825-1-AP | Download protocol |
| IHC protocol for SNAP23 antibody 10825-1-AP | Download protocol |
| IP protocol for SNAP23 antibody 10825-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Extracell Vesicles Identification of the SNARE complex that mediates the fusion of multivesicular bodies with the plasma membrane in exosome secretion
| ||
Aging Cell Knockdown of astrocytic Grin2a aggravates β-amyloid-induced memory and cognitive deficits through regulating nerve growth factor. | ||
Cell Death Differ Transglutaminase 2 crosslinks zona pellucida glycoprotein 3 to prevent polyspermy. | ||
Oncogene Small extracellular vesicles containing miR-30a-3p attenuate the migration and invasion of hepatocellular carcinoma by targeting SNAP23 gene.
| ||
Int J Mol Sci Exogenous H2S Attenuates Hypertension by Regulating Renin Exocytosis under Hyperglycaemic and Hyperlipidaemic Conditions |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Shweta (Verified Customer) (11-18-2024) | Antibody recognizes the correct band size and works great in WB
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