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Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using SNAP25 Polyclonal antibody (14903-1-AP)

    WB analysis of SH-SY5Y using 14903-1-AP (same clone as 14903-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14903-1-AP (SNAP25 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14903-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SNAP25 Polyclonal antibody (14903-1-AP)

    WB analysis using 14903-1-AP (same clone as 14903-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14903-1-AP (SNAP25 antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14903-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using SNAP25 Polyclonal antibody (14903-1-AP)

    WB analysis of mouse brain using 14903-1-AP (same clone as 14903-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14903-1-AP (SNAP25 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14903-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using SNAP25 Polyclonal antibody (14903-1-AP)

    WB analysis of rat brain using 14903-1-AP (same clone as 14903-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 14903-1-AP (SNAP25 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14903-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using SNAP25 Polyclonal antibody (14903-1-AP)

    IP experiment of mouse brain using 14903-1-AP (same clone as 14903-1-PBS)

    IP result of anti-SNAP25 (IP:14903-1-AP, 3ug; Detection:14903-1-AP 1:3500) with mouse brain tissue lysate 3600ug. This data was developed using the same antibody clone with 14903-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using SNAP25 Polyclonal antibody (14903-1-AP)

    IF Staining of SH-SY5Y using 14903-1-AP (same clone as 14903-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using SNAP25 antibody (14903-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14903-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using SNAP25 Polyclonal antibody (14903-1-AP)

    IF Staining of SH-SY5Y using 14903-1-AP (same clone as 14903-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using SNAP25 antibody (14903-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14903-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using SNAP25 Polyclonal antibody (14903-1-AP)

    IF Staining of PC-12 using 14903-1-AP (same clone as 14903-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using SNAP25 antibody (14903-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14903-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

SNAP25 Polyclonal antibody, PBS Only

SNAP25 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 14903-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14903-1-PBS targets SNAP25 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SNAP25 fusion protein Ag6695 相同性解析による交差性が予測される生物種
Full Name synaptosomal-associated protein, 25kDa
Calculated molecular weight 23 kDa
Observed molecular weight 25-27 kDa
GenBank accession numberBC010647
Gene Symbol SNAP25
Gene ID (NCBI) 6616
RRIDAB_2192051
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP60880
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

The synaptosomal associated protein of 25 kD (SNAP-25) was first identified as a major synaptic protein by Wilson and colleagues. The protein interacts with syntaxin and synaptobrevin through its N-terminal and C-terminal -helical domains. Its palmitoylation domain is located in the middle of the molecule that contains four cysteine residues. Mutation of the cysteines abolishes palmitoylation and membrane binding. Several elegant studies using synaptosome preparations and permeabilized PC12 cells have suggested that SNAP-25 may act in the late post-docking steps of exocytosis. By limited proteolysis and in vitro binding assay, it is proposed that the two helix domains act independently and contribute equally to form the SNARE complex with syntaxin and synaptobrevin. It seems that a major regulatory element is located in the C-terminus of SNAP-25. Removing a 9 amino acid sequence of SNAP-25 inhibited neurosecretion in chromaffin cells. In addition, it has been shown that inhibition of neurosecretion by AX type E can be rescued by a SNAP-25 C-terminal peptide, probably by initiating the formation of a fusion competent SNARE complex.