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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SNAP25 Monoclonal antibody, PBS Only

SNAP25 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3E4B7

Cat no : 60159-1-PBS

Synonyms

RIC 4, RIC4, SEC9, SNAP, SNAP 25, SNAP25, SUP, Super protein

Validation Data Gallery

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  • Western Blot (WB) analysis of pig brain tissue using SNAP25 Monoclonal antibody (60159-1-Ig)

    WB analysis of pig brain using 60159-1-Ig (same clone as 60159-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 60159-1-Ig (SNAP25 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60159-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using SNAP25 Monoclonal antibody (60159-1-Ig)

    WB analysis of rat brain using 60159-1-Ig (same clone as 60159-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 60159-1-Ig (SNAP25 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60159-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using SNAP25 Monoclonal antibody (60159-1-Ig)

    WB analysis of mouse brain using 60159-1-Ig (same clone as 60159-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 60159-1-Ig (SNAP25 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60159-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of fetal human brain tissue using SNAP25 Monoclonal antibody (60159-1-Ig)

    WB analysis of fetal human brain using 60159-1-Ig (same clone as 60159-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60159-1-Ig (SNAP25 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60159-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-12 cells using SNAP25 Monoclonal antibody (60159-1-Ig)

    WB analysis of PC-12 using 60159-1-Ig (same clone as 60159-1-PBS)

    PC-12 cells were subjected to SDS PAGE followed by western blot with 60159-1-Ig (SNAP25 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60159-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using SNAP25 Monoclonal antibody (60159-1-Ig)

    WB analysis of HEK-293 using 60159-1-Ig (same clone as 60159-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 60159-1-Ig (SNAP25 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60159-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using SNAP25 Monoclonal antibody (60159-1-Ig)

    IP experiment of mouse brain using 60159-1-Ig (same clone as 60159-1-PBS)

    IP result of anti-SNAP25 (IP:60159-1-Ig, 3ug; Detection:60159-1-Ig 1:500) with mouse brain tissue lysate 3600ug. This data was developed using the same antibody clone with 60159-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using SNAP25 Monoclonal antibody (60159-1-Ig)

    IHC staining of rat brain using 60159-1-Ig (same clone as 60159-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 60159-1-Ig (SNAP25 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60159-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using SNAP25 Monoclonal antibody (60159-1-Ig)

    IHC staining of rat brain using 60159-1-Ig (same clone as 60159-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 60159-1-Ig (SNAP25 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60159-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SNAP25 Monoclonal antibody (60159-1-Ig)

    IHC staining of mouse brain using 60159-1-Ig (same clone as 60159-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60159-1-Ig (SNAP25 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60159-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SNAP25 Monoclonal antibody (60159-1-Ig)

    IHC staining of mouse brain using 60159-1-Ig (same clone as 60159-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60159-1-Ig (SNAP25 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60159-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using SNAP25 Monoclonal antibody (60159-1-Ig)

    IF Staining of PC-12 using 60159-1-Ig (same clone as 60159-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using SNAP25 antibody (60159-1-Ig, Clone: 3E4B7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60159-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using SNAP25 Monoclonal antibody (60159-1-Ig)

    IF Staining of PC-12 using 60159-1-Ig (same clone as 60159-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using SNAP25 antibody (60159-1-Ig, Clone: 3E4B7 ) at dilution of 1:1000 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L), SNAP25 antibody (14903-1-AP, green). This data was developed using the same antibody clone with 60159-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60159-1-PBS targets SNAP25 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SNAP25 fusion protein Ag6695 相同性解析による交差性が予測される生物種
Full Name synaptosomal-associated protein, 25kDa
Calculated molecular weight 23 kDa
Observed molecular weight 25-30 kDa
GenBank accession numberBC010647
Gene symbol SNAP25
Gene ID (NCBI) 6616
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The synaptosomal associated protein of 25 kD (SNAP-25) was first identified as a major synaptic protein by Wilson and colleagues. The protein interacts with syntaxin and synaptobrevin through its N-terminal and C-terminal -helical domains. Its palmitoylation domain is located in the middle of the molecule that contains four cysteine residues. Mutation of the cysteines abolishes palmitoylation and membrane binding. Several elegant studies using synaptosome preparations and permeabilized PC12 cells have suggested that SNAP-25 may act in the late post-docking steps of exocytosis. By limited proteolysis and in vitro binding assay, it is proposed that the two helix domains act independently and contribute equally to form the SNARE complex with syntaxin and synaptobrevin. It seems that a major regulatory element is located in the C-terminus of SNAP-25. Removing a 9 amino acid sequence of SNAP-25 inhibited neurosecretion in chromaffin cells.