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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SNAP91 Polyclonal antibody (14093-1-AP)

    WB analysis using 14093-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14093-1-AP (SNAP91 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SNAP91 Polyclonal antibody (14093-1-AP)

    IHC staining of mouse brain using 14093-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14093-1-AP (SNAP91 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SNAP91 Polyclonal antibody (14093-1-AP)

    IHC staining of mouse brain using 14093-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14093-1-AP (SNAP91 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using SNAP91 Polyclonal antibody (14093-1-AP)

    IF Staining of mouse brain using 14093-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using SNAP91 antibody (14093-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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SNAP91 Polyclonal antibody

SNAP91 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 14093-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, ELISA

Synonyms

Phosphoprotein F1-20, KIAA0656, Clathrin coat-associated protein AP180, Clathrin coat assembly protein AP180, 91 kDa synaptosomal-associated protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

14093-1-AP targets SNAP91 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitypig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SNAP91 fusion protein Ag5224 相同性解析による交差性が予測される生物種
Full Name synaptosomal-associated protein, 91kDa homolog (mouse)
Calculated molecular weight92.5 kDa
Observed molecular weight160 kDa
GenBank accession numberBC060818
Gene Symbol SNAP91
Gene ID (NCBI) 9892
RRIDAB_2923560
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60641
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SNAP91, also known as AP180 or CALM, is a clathrin-coat assembly protein that sequesters clathrin. SNAP91/AP180 is required for clathrin-dependent endocytosis and SV reformation (PMID: 26903854; PMID: 31843760).The AP180 N-terminal homology (ANTH) domains is crucially involved in membrane budding processes, along with and Epsin N-terminal homology (ENTH) domains (PMID: 29774507).

Protocols

Product Specific Protocols
WB protocol for SNAP91 antibody 14093-1-APDownload protocol
IHC protocol for SNAP91 antibody 14093-1-APDownload protocol
IF protocol for SNAP91 antibody 14093-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigWB

Emerg Microbes Infect

CD1d facilitates African swine fever virus entry into the host cells via clathrin-mediated endocytosis

Authors - Xin Chen
View Article