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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SNAT2 Polyclonal antibody

SNAT2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25928-1-AP

Synonyms

SLC38A2, SAT2, Protein 40-9-1, Protein 40 9 1, PRO1068

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SNAT2 Polyclonal antibody (25928-1-AP)

    WB analysis using 25928-1-AP

    37°C incubated and boiled mouse brain tissues were subjected to SDS PAGE followed by western blot with 25928-1-AP (SNAT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SNAT2 Polyclonal antibody (25928-1-AP)

    IHC staining of human kidney using 25928-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25928-1-AP (SNAT2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using SNAT2 Polyclonal antibody (25928-1-AP)

    IHC staining of human thyroid cancer using 25928-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 25928-1-AP (SNAT2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SNAT2 Polyclonal antibody (25928-1-AP)

    IHC staining of mouse kidney using 25928-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 25928-1-AP (SNAT2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat pancreas tissue using SNAT2 Polyclonal antibody (25928-1-AP)

    IHC staining of rat pancreas using 25928-1-AP

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 25928-1-AP (SNAT2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using SNAT2 Polyclonal antibody (25928-1-AP)

    IHC staining of human cervical cancer using 25928-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 25928-1-AP (SNAT2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using SNAT2 Polyclonal antibody (25928-1-AP)

    IHC staining of rat kidney using 25928-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 25928-1-AP (SNAT2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using SNAT2 Polyclonal antibody (25928-1-AP)

    IHC staining of mouse pancreas using 25928-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 25928-1-AP (SNAT2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using SNAT2 Polyclonal antibody (25928-1-AP)

    IHC staining of mouse pancreas using 25928-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 25928-1-AP (SNAT2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected in37°C incubated mouse brain tissue
Positive IHC detected inmouse pancreas tissue, human cervical cancer tissue, human kidney tissue, rat pancreas tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25928-1-AP targets SNAT2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SNAT2 fusion protein Ag23082 相同性解析による交差性が予測される生物種
Full Name solute carrier family 38, member 2
Observed molecular weight45 kDa, 56 kDa
GenBank accession numberBC040342
Gene symbol SLC38A2
Gene ID (NCBI) 54407
RRIDAB_3085830
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SNAT2 (Sodium-dependent neutral amino acid transporter-2), the ubiquitous member of SLC38 family, accounts for the activity of transport system A for neutral amino acids in most mammalian tissues (PMID: 16734764). SNAT2 mediates uptake of neutral α-amino acids and are expressed in central neurons (PMID: 19240036). SNAT2 is expressed in breast cancer cell lines. SLC38A2 also acts as a selective target for inhibiting growth of Gln-dependent breast cancer cell lines (PMID: 33028955).

Protocols

Product Specific Protocols
WB protocol for SNAT2 antibody 25928-1-APDownload protocol
IHC protocol for SNAT2 antibody 25928-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols