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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SNCAIP Polyclonal antibody

SNCAIP Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17818-1-AP

Synonyms

SNCAIP, Sph1, Synphilin 1, SYPH1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using SNCAIP Polyclonal antibody (17818-1-AP)

    WB analysis of mouse brain using 17818-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17818-1-AP (SNCAIP Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of fetal human brain tissue using SNCAIP Polyclonal antibody (17818-1-AP)

    WB analysis of fetal human brain using 17818-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 17818-1-AP (SNCAIP Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using SNCAIP Polyclonal antibody (17818-1-AP)

    IP experiment of mouse brain using 17818-1-AP

    IP result of anti-SNCAIP (IP:17818-1-AP, 4ug; Detection:17818-1-AP 1:300) with mouse brain tissue lysate 3600ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SNCAIP Polyclonal antibody (17818-1-AP)

    IHC staining of human kidney using 17818-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17818-1-AP (SNCAIP Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using SNCAIP Polyclonal antibody (17818-1-AP)

    IHC staining of human kidney using 17818-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17818-1-AP (SNCAIP Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

Positive WB detected inmouse brain tissue, fetal human brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 17818-1-AP is SNCAIP Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SNCAIP fusion protein Ag12147 相同性解析による交差性が予測される生物種
Full Name synuclein, alpha interacting protein
Calculated molecular weight603aa,67 kDa; 1016aa,111 kDa
Observed molecular weight 100 kDa, 75 kDa
GenBank accession numberBC094759
Gene symbol SNCAIP
Gene ID (NCBI) 9627
RRIDAB_2878444
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SNCAIP, also named as Synphilin-1 or Alpha-synuclein-interacting protein, is a 919 amino acid protein, which contains six ANK repeats. SNCAIP is detected in brain and is widely expressed, with highest levels in brain, heart and placenta. SNCAIP localizes in the cytoplasm and inhibits the ubiquitin ligase activity of SIAH1 and inhibits proteasomal degradation of target proteins. SNCAIP has an association with Parkinson disease.

Protocols

Product Specific Protocols
WB protocol for SNCAIP antibody 17818-1-APDownload protocol
IHC protocol for SNCAIP antibody 17818-1-APDownload protocol
IP protocol for SNCAIP antibody 17818-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Neurosci Lett

Synphilin-1 has neuroprotective effects on MPP+-induced Parkinson's disease model cells by inhibiting ROS production and apoptosis.

Authors - Takeo Shishido
View Article