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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SNCB Monoclonal antibody (68046-1-Ig)

    WB analysis using 68046-1-Ig

    Brain tissues were subjected to SDS PAGE followed by western blot with 68046-1-Ig (SNCB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using SNCB Monoclonal antibody (68046-1-Ig)

    IF Staining of mouse brain using 68046-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SNCB antibody (68046-1-Ig, Clone: 2B10E10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

SNCB Monoclonal antibody

SNCB Monoclonal Antibody for WB, IF-P, ELISA
Cat No. 68046-1-Ig
Clone No.2B10E10

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, rabbit, chicken

Applications

WB, IF-P, ELISA

Synonyms

Beta synuclein, SNCB, synuclein, beta

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inpig brain tissue, rabbit brain, rat brain, mouse brain, chicken brain
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68046-1-Ig targets SNCB in WB, IF-P, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit, chicken samples.

Tested Reactivity Human, mouse, rat, pig, rabbit, chicken
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SNCB fusion protein Ag0772 相同性解析による交差性が予測される生物種
Full Name synuclein, beta
Calculated molecular weight 14 kDa
Observed molecular weight18 kDa
GenBank accession numberBC002902
Gene Symbol SNCB
Gene ID (NCBI) 6620
RRIDAB_2918788
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ16143
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SNCB antibody 68046-1-IgDownload protocol
IF protocol for SNCB antibody 68046-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols