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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SNCG Polyclonal antibody

SNCG Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16398-1-AP

Synonyms

Gamma synuclein, Persyn, PRSN, SNCG, SR, Synoretin

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SNCG Polyclonal antibody (16398-1-AP)

    WB analysis using 16398-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16398-1-AP (SNCG antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SNCG Polyclonal antibody (16398-1-AP)

    IHC staining of mouse brain using 16398-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16398-1-AP (SNCG antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SNCG Polyclonal antibody (16398-1-AP)

    IHC staining of mouse brain using 16398-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16398-1-AP (SNCG antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHT-29 cells, HeLa cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16398-1-AP targets SNCG in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SNCG fusion protein Ag9493 相同性解析による交差性が予測される生物種
Full Name synuclein, gamma (breast cancer-specific protein 1)
Calculated molecular weight 127 aa, 13 kDa
Observed molecular weight13 kDa
GenBank accession numberBC014098
Gene symbol SNCG
Gene ID (NCBI) 6623
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Gamma synuclein, also known as SNCG, BCSG1, belongs to the synuclein family. SNCG is a synuclein protein found primarily in the peripheral nervous system and retina(PMID 11806835). SNCG is the least conserved of the synuclein proteins and is detected in the brain, ovarian tumors, and the olfactory epithelium(PMID 9750188). SNCG is expressed in high abundance in breast cancer but scarcely at all in a normal breast and is considered a breast cancer marker(PMID: 10813729).

Protocols

Product Specific Protocols
WB protocol for SNCG antibody 16398-1-APDownload protocol
IHC protocol for SNCG antibody 16398-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols