- Featured Product
- KD/KO Validated
SND1 Polyclonal antibody
SND1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IHC, IF/ICC, ChIP, RIP, ELISA
Conjugate
Unconjugated
Cat no : 10760-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, Jurkat cells, COLO 320 cells, HeLa cells |
| Positive IHC detected in | human breast hyperplasia tissue, human breast cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 6 publications below |
| IHC | See 3 publications below |
| IF | See 2 publications below |
| ChIP | See 1 publications below |
| RIP | See 2 publications below |
Product Information
10760-1-AP targets SND1 in WB, IHC, IF/ICC, ChIP, RIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SND1 fusion protein Ag1200 相同性解析による交差性が予測される生物種 |
| Full Name | staphylococcal nuclease and tudor domain containing 1 |
| Calculated molecular weight | 101 kDa |
| Observed molecular weight | 101 kDa |
| GenBank accession number | BC017180 |
| Gene symbol | SND1 |
| Gene ID (NCBI) | 27044 |
| RRID | AB_2193410 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Staphylococcal nuclease domain-containing 1 (SND1), is a multifunctional nuclease that consists of four staphylococcal nuclease domains and a tudor domain. SND1 acts as a coactivator that facilitates transcriptional activity of STAT5, 6 and c-Myc. SND1 is a comprising part of the RNA-induced silencing complex(RISC), and takes part in the functions of miRNA, regulates transcription through transcriptional coactivation, RNA interference, RNA splicing, and RNA editing. Higher level of SND1 has been found in colon cancer and prostate cancer, can promote HCC angiogenesis in xenograft model through induction of angiogenic factors. This is a rabbit polyclonal antibody raised against near the C-terminus of human SND1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SND1 antibody 10760-1-AP | Download protocol |
| IHC protocol for SND1 antibody 10760-1-AP | Download protocol |
| IF protocol for SND1 antibody 10760-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell SND1 binds SARS-CoV-2 negative-sense RNA and promotes viral RNA synthesis through NSP9 | ||
Cell Res Predicting dynamic cellular protein-RNA interactions by deep learning using in vivo RNA structures. | ||
Elife The Tudor SND1 protein is an m6A RNA reader essential for replication of Kaposi's sarcoma-associated herpesvirus.
| ||
J Cell Physiol The phosphorylation of Tudor-SN mediated by JNK is involved in the regulation of milk protein synthesis induced by prolactin in BMECs. |
