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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using SND1 Monoclonal antibody (60265-1-Ig)

    WB analysis of Jurkat using 60265-1-Ig (same clone as 60265-1-PBS)

    WB result of SND1 antibody (60265-1-Ig, 1:60,000) with si-Control and si-SND1 transfected Jurkat cells. This data was developed using the same antibody clone with 60265-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SND1 Monoclonal antibody (60265-1-Ig)

    WB analysis using 60265-1-Ig (same clone as 60265-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60265-1-Ig (SND1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60265-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SND1 Monoclonal antibody (60265-1-Ig)

    WB analysis using 60265-1-Ig (same clone as 60265-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60265-1-Ig (SND1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60265-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using SND1 Monoclonal antibody (60265-1-Ig)

    WB analysis of HeLa using 60265-1-Ig (same clone as 60265-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 60265-1-Ig (SND1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60265-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using SND1 Monoclonal antibody (60265-1-Ig)

    IP experiment of HeLa using 60265-1-Ig (same clone as 60265-1-PBS)

    IP result of anti-SND1 (IP:60265-1-Ig, 5ug; Detection:60265-1-Ig 1:500) with HeLa cells lysate 1400ug. This data was developed using the same antibody clone with 60265-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SND1 Monoclonal antibody (60265-1-Ig)

    IHC staining of human colon cancer using 60265-1-Ig (same clone as 60265-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60265-1-Ig (SND1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60265-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using SND1 Monoclonal antibody (60265-1-Ig)

    IHC staining of human pancreas using 60265-1-Ig (same clone as 60265-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 60265-1-Ig (SND1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60265-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SND1 Monoclonal antibody (60265-1-Ig)

    IHC staining of human colon cancer using 60265-1-Ig (same clone as 60265-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60265-1-Ig (SND1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60265-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast hyperplasia tissue using SND1 Monoclonal antibody (60265-1-Ig)

    IHC staining of human breast hyperplasia using 60265-1-Ig (same clone as 60265-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60265-1-Ig (SND1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60265-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast hyperplasia tissue using SND1 Monoclonal antibody (60265-1-Ig)

    IHC staining of human breast hyperplasia using 60265-1-Ig (same clone as 60265-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60265-1-Ig (SND1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60265-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SND1 Monoclonal antibody (60265-1-Ig)

    IHC staining of human breast cancer using 60265-1-Ig (same clone as 60265-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60265-1-Ig (SND1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60265-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using SND1 Monoclonal antibody (60265-1-Ig)

    IHC staining of human pancreas using 60265-1-Ig (same clone as 60265-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 60265-1-Ig (SND1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60265-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SND1 Monoclonal antibody (60265-1-Ig)

    IHC staining of human breast cancer using 60265-1-Ig (same clone as 60265-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60265-1-Ig (SND1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60265-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SND1 Monoclonal antibody (60265-1-Ig)

    IF Staining of HepG2 using 60265-1-Ig (same clone as 60265-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 60265-1-Ig(SND1 antibody) at dilution of 1:50 and and Rhodamine-labeled goat anti-mouse IgG (red). This data was developed using the same antibody clone with 60265-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SND1 Monoclonal antibody (60265-1-Ig)

    IF Staining of HepG2 using 60265-1-Ig (same clone as 60265-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SND1 antibody (60265-1-Ig, Clone: 1A6A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60265-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

SND1 Monoclonal antibody, PBS Only

SND1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 60265-1-PBS
Clone No.1A6A4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

100 kDa coactivator, EBNA2 coactivator p100, p100, p100 co activator, SND1, TDRD11

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60265-1-PBS targets SND1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SND1 fusion protein Ag1200 相同性解析による交差性が予測される生物種
Full Name staphylococcal nuclease and tudor domain containing 1
Calculated molecular weight 101 kDa
Observed molecular weight 101 kDa
GenBank accession numberBC017180
Gene Symbol SND1
Gene ID (NCBI) 27044
RRIDAB_2881386
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ7KZF4
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Staphylococcal nuclease domain-containing 1 (SND1), is a multifunctional nuclease that consists of four staphylococcal nuclease domains and a tudor domain. SND1 acts as a coactivator that facilitates transcriptional activity of STAT5, 6 and c-Myc. SND1 is a comprising part of the RNA-induced silencing complex(RISC), and takes part in the functions of miRNA, regulates transcription through transcriptional coactivation, RNA interference, RNA splicing, and RNA editing. Higher level of SND1 has been found in colon cancer and prostate cancer, can promote HCC angiogenesis in xenograft model through induction of angiogenic factors.