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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SNF2L Polyclonal antibody

SNF2L Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29461-1-AP

Synonyms

ATP dependent helicase SMARCA1, DKFZp686D1623, FLJ41547, ISWI, NURF140, SMARCA1, SNF2L, SNF2L1, SNF2LB, SWI, SWI2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SNF2L Polyclonal antibody (29461-1-AP)

    WB analysis using 29461-1-AP

    U-87 MG cells were subjected to SDS PAGE followed by western blot with 29461-1-AP (SNF2L antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-251 cells using SNF2L Polyclonal antibody (29461-1-AP)

    WB analysis of U-251 using 29461-1-AP

    U-251 cells were subjected to SDS PAGE followed by western blot with 29461-1-AP (SNF2L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using SNF2L Polyclonal antibody (29461-1-AP)

    WB analysis of SH-SY5Y using 29461-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 29461-1-AP (SNF2L antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat lung tissue using SNF2L Polyclonal antibody (29461-1-AP)

    IHC staining of rat lung using 29461-1-AP

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 29461-1-AP (SNF2L antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using SNF2L Polyclonal antibody (29461-1-AP)

    IHC staining of mouse lung using 29461-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 29461-1-AP (SNF2L antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inU-87 MG cells, U-251 cells, SH-SY5Y cells
Positive IHC detected inmouse lung tissue, rat lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

29461-1-AP targets SNF2L in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SNF2L fusion protein Ag30657 相同性解析による交差性が予測される生物種
Full Name SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 1
Calculated molecular weight 123 kDa
Observed molecular weight120-130 KDa
GenBank accession numberNM_003069
Gene symbol SMARCA1/SNF2L
Gene ID (NCBI) 6594
RRIDAB_2918310
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SNF2L, also named as SMARCA1, NURF140 and ISWI, encodes the SWI/SNF family of proteins. The encoded protein is an ATPase which is expressed in diverse tissues and contributes to the chromatin remodeling complex that is involved in transcription (PMID: 28801535). SNF2L was shown to promote neurite outgrowth as well as regulate the human engrailed homeotic genes, important regulators of brain development (PMID: 15310751). The protein may also play a role in DNA damage, growth inhibition and apoptosis of cancer cells (PMID: 25462860). SNF2L has 2 isoforms with MW of 123 kDa and 121 kDa, and can be detected as 120-130 kDa.

Protocols

Product Specific Protocols
WB protocol for SNF2L antibody 29461-1-APDownload protocol
IHC protocol for SNF2L antibody 29461-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Differ

BAP18 facilitates CTCF-mediated chromatin accessible to regulate enhancer activity in breast cancer

Authors - Ge Sun
View Article