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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SNF8 Monoclonal antibody, PBS Only

SNF8 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C10F2

Cat no : 67696-1-PBS

Synonyms

Dot3, EAP30, ESCRT II complex subunit VPS22, hVps22, SNF8, Vacuolar sorting protein SNF8, VPS22

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SNF8 Monoclonal antibody (67696-1-Ig)

    WB analysis using 67696-1-Ig (same clone as 67696-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67696-1-Ig (SNF8 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67696-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SNF8 Monoclonal antibody (67696-1-Ig)

    IHC staining of human breast cancer using 67696-1-Ig (same clone as 67696-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67696-1-Ig (SNF8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67696-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SNF8 Monoclonal antibody (67696-1-Ig)

    IHC staining of human breast cancer using 67696-1-Ig (same clone as 67696-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67696-1-Ig (SNF8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67696-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SNF8 Monoclonal antibody (67696-1-Ig)

    IF Staining of HeLa using 67696-1-Ig (same clone as 67696-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SNF8 antibody (67696-1-Ig, Clone: 2C10F2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67696-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67696-1-PBS targets SNF8 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SNF8 fusion protein Ag30384 相同性解析による交差性が予測される生物種
Full Name SNF8, ESCRT-II complex subunit, homolog (S. cerevisiae)
Calculated molecular weight 258 aa, 29 kDa
Observed molecular weight29 kDa
GenBank accession numberBC008976
Gene symbol SNF8
Gene ID (NCBI) 11267
RRIDAB_2882888
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.