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RCC1 Polyclonal antibody

RCC1 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 10697-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

RCC1, SNHG3 RCC1, SNHG3-RCC1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inA431 cells, Jurkat cells, mouse testis tissue, Raji cells, SGC-7901 cells, HeLa cells, HEK-293 cells, HEK-293T cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman ovary tumor tissue, human urothelial carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10697-1-AP targets RCC1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RCC1 fusion protein Ag1042 相同性解析による交差性が予測される生物種
Full Name regulator of chromosome condensation 1
Calculated molecular weight 45 kDa, 48 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC007300
Gene Symbol RCC1
Gene ID (NCBI) 1104
RRIDAB_513962
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP18754
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CHC1, also named as RCC1, SNHG3-RCC1, promotes the exchange of ran-bound gdp by gtp. It is involved in the regulation of onset of chromosome condensation in the S-phase. Phosphorylation of RCC1 on serines located in or near its nuclear localization signal activates RCC1 to generate RanGTP on mitotic chromosomes, which is required for spindle assembly and chromosome segregation. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human RCC1. The geneID has updated as 1104 recently.

Protocols

Product Specific Protocols
WB protocol for RCC1 antibody 10697-1-APDownload protocol
IHC protocol for RCC1 antibody 10697-1-APDownload protocol
IP protocol for RCC1 antibody 10697-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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