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  • Featured Product
  • KD/KO Validated

RCC1 Polyclonal antibody

RCC1 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 10697-1-AP

Synonyms

RCC1, SNHG3 RCC1, SNHG3-RCC1

Validation Data Gallery

Applications:
  • WB analysis of HEK-293 using 10697-1-AP

    WB analysis of HEK-293 using 10697-1-AP

    WB result of RCC1 antibody (10697-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RCC1 transfected HEK-293 cells.

  • WB analysis using 10697-1-AP

    WB analysis using 10697-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10697-1-AP (RCC1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 10697-1-AP

    WB analysis of Jurkat using 10697-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10697-1-AP (RCC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 10697-1-AP

    WB analysis of HeLa using 10697-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10697-1-AP (RCC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of SGC-7901 using 10697-1-AP

    WB analysis of SGC-7901 using 10697-1-AP

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 10697-1-AP (RCC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of SGC-7901 using 10697-1-AP

    WB analysis of SGC-7901 using 10697-1-AP

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 10697-1-AP (RCC1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse testis using 10697-1-AP

    WB analysis of mouse testis using 10697-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 10697-1-AP (RCC1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • WB analysis of Raji using 10697-1-AP

    WB analysis of Raji using 10697-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 10697-1-AP (RCC1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 10697-1-AP

    IP experiment of HeLa using 10697-1-AP

    IP result of anti-RCC1 (IP:10697-1-AP, 4ug; Detection:10697-1-AP 1:500) with HeLa cells lysate 1600 ug.

  • IHC staining of human ovary tumor using 10697-1-AP

    IHC staining of human ovary tumor using 10697-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10697-1-AP (RCC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary tumor using 10697-1-AP

    IHC staining of human ovary tumor using 10697-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10697-1-AP (RCC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human urothelial carcinoma using 10697-1-AP

    IHC staining of human urothelial carcinoma using 10697-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10697-1-AP (RCC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human urothelial carcinoma using 10697-1-AP

    IHC staining of human urothelial carcinoma using 10697-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10697-1-AP (RCC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inA431 cells, Jurkat cells, mouse testis tissue, Raji cells, SGC-7901 cells, HeLa cells, HEK-293 cells, HEK-293T cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman ovary tumor tissue, human urothelial carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10697-1-AP targets RCC1 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RCC1 fusion protein Ag1042 相同性解析による交差性が予測される生物種
Full Name SNHG3-RCC1 readthrough transcript
Calculated molecular weight 45 kDa, 48 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC007300
Gene symbol SNHG3-RCC1
Gene ID (NCBI) 751867
RRIDAB_513962
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CHC1, also named as RCC1, SNHG3-RCC1, promotes the exchange of ran-bound gdp by gtp. It is involved in the regulation of onset of chromosome condensation in the S-phase. Phosphorylation of RCC1 on serines located in or near its nuclear localization signal activates RCC1 to generate RanGTP on mitotic chromosomes, which is required for spindle assembly and chromosome segregation. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human RCC1. The geneID has updated as 1104 recently.

Protocols

Product Specific Protocols
WB protocol for RCC1 antibody 10697-1-APDownload protocol
IHC protocol for RCC1 antibody 10697-1-APDownload protocol
IP protocol for RCC1 antibody 10697-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols