Validation Data Gallery
Tested Applications
Positive IHC detected in | mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
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Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
19890-1-AP targets SNRNP25 in IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | SNRNP25 fusion protein Ag13724 相同性解析による交差性が予測される生物種 |
Full Name | small nuclear ribonucleoprotein 25kDa (U11/U12) |
Calculated molecular weight | 132 aa, 15 kDa |
GenBank accession number | BC001381 |
Gene Symbol | SNRNP25 |
Gene ID (NCBI) | 79622 |
RRID | AB_3085590 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9BV90 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SNRNP25, small nuclear ribonucleoprotein U11/U12 subunit 25, encodes a 25K protein that is a component of the U12-type spliceosome. Two types of spliceosomes catalyze splicing of pre-mRNAs. The major U2-type spliceosome is found in all eukaryotes and removes U2-type introns, which represent more than 99% of pre-mRNA introns. The U12-type spliceosome consists of several small nuclear RNAs and associated proteins. LRP1-SNRNP25 fusion gene promotes osteosarcoma cell invasion and migration (PMID: 26738504, PMID: 25300797).
Protocols
Product Specific Protocols | |
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IHC protocol for SNRNP25 antibody 19890-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |