Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of HeLa using 13512-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 13512-1-AP (SNRPB2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 13512-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 13512-1-AP (SNRPB2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 13512-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 13512-1-AP (SNRPB2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 13512-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 13512-1-AP (SNRPB2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 13512-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 13512-1-AP (SNRPB2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 13512-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 13512-1-AP (SNRPB2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 13512-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 13512-1-AP (SNRPB2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 13512-1-AP
IP result of anti-SNRPB2 (IP:13512-1-AP, 4ug; Detection:13512-1-AP 1:1000) with mouse liver tissue lysate 4000ug.
IHC staining of human cervical cancer using 13512-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13512-1-AP (SNRPB2 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human cervical cancer using 13512-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13512-1-AP (SNRPB2 antibody) at dilution of 1:100 (under 10x lens).
IF Staining of HeLa using 13512-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13512-1-AP (SNRPB2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 13512-1-AP
Immunofluorescent analysis of Hela cells, using SNRPB2 antibody 13512-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Featured Product
KD/KO Validated

SNRPB2 Polyclonal antibody

SNRPB2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 13512-1-AP

Publications(10)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Synonyms

U2 snRNP B'', U2 snRNP B, U2 small nuclear ribonucleoprotein B''

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (10)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, human brain tissue, human kidney tissue, human liver tissue, mouse liver tissue, mouse lung tissue
Positive IP detected in	mouse liver tissue
Positive IHC detected in	human cervical cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 8 publications below
IHC	See 2 publications below
IF	See 3 publications below
IP	See 1 publications below
RIP	See 1 publications below

Product Information

13512-1-AP targets SNRPB2 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SNRPB2 fusion protein Ag4436 相同性解析による交差性が予測される生物種
Full Name	small nuclear ribonucleoprotein polypeptide B''
Calculated molecular weight	225 aa, 26, 5 kDa
Observed molecular weight	25-31 kDa
GenBank accession number	BC036737
Gene Symbol	SNRPB2
Gene ID (NCBI)	6629
RRID	AB_2302390
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P08579
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

U2 small nuclear ribonucleoprotein B''(SNRPB2) is part of the U2 RNP particle, which associated with the branch point of the lariat-shaped intron, in intermediate in the cascade of mRNA precursor splicing event. Only in presence of the U2A' protein, SNRPB2 binds stem loop IV of U2 snRNA.

Protocols

Product Specific Protocols
WB protocol for SNRPB2 antibody 13512-1-AP	Download protocol
IHC protocol for SNRPB2 antibody 13512-1-AP	Download protocol
IF protocol for SNRPB2 antibody 13512-1-AP	Download protocol
IP protocol for SNRPB2 antibody 13512-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nature CPEB1 coordinates alternative 3'-UTR formation with translational regulation. Authors - Felice-Alessio Bava View Article
human	WB,IHC	Sci Transl Med Comment on "Drug screening for ALS using patient-specific induced pluripotent stem cells". Authors - Bilada Bilican View Article
human	IF	Sci Transl Med Drug screening for ALS using patient-specific induced pluripotent stem cells. Authors - Naohiro Egawa View Article
human	WB	Nucleic Acids Res Stoichiometries of U2AF35, U2AF65 and U2 snRNP reveal new early spliceosome assembly pathways. Authors - Li Chen View Article
human	WB	Cell Chem Biol Novel RNA-Affinity Proteogenomics Dissects Tumor Heterogeneity for Revealing Personalized Markers in Precision Prognosis of Cancer. Authors - Li Wang View Article
human	WB	STAR Protoc Protocol for proteogenomic dissection of intronic splicing enhancer interactome for prediction of individualized cancer prognosis. Authors - Li Wang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 13512-1-AP

WB analysis of mouse lung using 13512-1-AP

WB analysis of human kidney using 13512-1-AP

WB analysis of human liver using 13512-1-AP

WB analysis of human brain using 13512-1-AP

WB analysis of mouse liver using 13512-1-AP

WB analysis of HeLa using 13512-1-AP

IP experiment of mouse liver using 13512-1-AP

IHC staining of human cervical cancer using 13512-1-AP

IHC staining of human cervical cancer using 13512-1-AP

IF Staining of HeLa using 13512-1-AP

IF Staining of HeLa using 13512-1-AP

SNRPB2 Polyclonal antibody

SNRPB2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

CPEB1 coordinates alternative 3'-UTR formation with translational regulation.

Comment on "Drug screening for ALS using patient-specific induced pluripotent stem cells".

Drug screening for ALS using patient-specific induced pluripotent stem cells.

Stoichiometries of U2AF35, U2AF65 and U2 snRNP reveal new early spliceosome assembly pathways.

Novel RNA-Affinity Proteogenomics Dissects Tumor Heterogeneity for Revealing Personalized Markers in Precision Prognosis of Cancer.

Protocol for proteogenomic dissection of intronic splicing enhancer interactome for prediction of individualized cancer prognosis.