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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SNRPB2 Monoclonal antibody

SNRPB2 Monoclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1D11E9

Cat no : 68095-1-Ig

Synonyms

U2 snRNP B'', U2 snRNP B, U2 small nuclear ribonucleoprotein B'', 1D11E9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SNRPB2 Monoclonal antibody (68095-1-Ig)

    WB analysis using 68095-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68095-1-Ig (SNRPB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • Flow cytometry (FC) experiment of HeLa cells using SNRPB2 Monoclonal antibody (68095-1-Ig)

    FC experiment of HeLa using 68095-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug SNRPB2 Monoclonal antibody (68095-1-Ig, Clone:1D11E9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68095-1-Ig targets SNRPB2 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SNRPB2 fusion protein Ag5540 相同性解析による交差性が予測される生物種
Full Name small nuclear ribonucleoprotein polypeptide B''
Calculated molecular weight 225 aa, 26, 5 kDa
Observed molecular weight25-31 kDa
GenBank accession numberBC036737
Gene symbol SNRPB2
Gene ID (NCBI) 6629
RRIDAB_2918832
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

U2 small nuclear ribonucleoprotein B''(SNRPB2) is part of the U2 RNP particle, which associated with the branch point of the lariat-shaped intron, in intermediate in the cascade of mRNA precursor splicing event. Only in presence of the U2A' protein, SNRPB2 binds stem loop IV of U2 snRNA.

Protocols

Product Specific Protocols
WB protocol for SNRPB2 antibody 68095-1-IgDownload protocol
FC protocol for SNRPB2 antibody 68095-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols