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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using SNRPD2 Monoclonal antibody (66111-1-Ig)

    WB analysis of HeLa using 66111-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66111-1-Ig (SNRPD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using SNRPD2 Monoclonal antibody (66111-1-Ig)

    WB analysis of MCF-7 using 66111-1-Ig

    MCF7 cells were subjected to SDS PAGE followed by western blot with 66111-1-Ig (SNRPD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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SNRPD2 Monoclonal antibody

SNRPD2 Monoclonal Antibody for WB, ELISA
Cat No. 66111-1-Ig
Clone No.1F12B2

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, ELISA

Synonyms

Sm D2, SMD2, snRNP core protein D2, SNRPD2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHela cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66111-1-Ig targets SNRPD2 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SNRPD2 fusion protein Ag7115 相同性解析による交差性が予測される生物種
Full Name small nuclear ribonucleoprotein D2 polypeptide 16.5kDa
Calculated molecular weight 14 kDa
Observed molecular weight 14-16 kDa
GenBank accession numberBC000486
Gene Symbol SNRPD2
Gene ID (NCBI) 6633
RRIDAB_2881510
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP62316
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Systemic lupus erythematosus is characterized by antibodies to a variety of intracellular self-antigens, such as dsDNA and Sm, and these serve as hallmarks in the diagnosis of systemic autoimmune diseases. SNRPD2 is one of Sm protein, and is required for pre-mRNA splicing, snRNP biogenesis.

Protocols

Product Specific Protocols
WB protocol for SNRPD2 antibody 66111-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols