• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SNRPE Polyclonal antibody

SNRPE Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20407-1-AP

Synonyms

Sm E, Sm protein E, SME, snRNP E, SNRPE

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using SNRPE Polyclonal antibody (20407-1-AP)

    WB analysis using 20407-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20407-1-AP (SNRPE antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using SNRPE Polyclonal antibody (20407-1-AP)

    WB analysis of human placenta using 20407-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 20407-1-AP (SNRPE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using SNRPE Polyclonal antibody (20407-1-AP)

    WB analysis of human brain using 20407-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 20407-1-AP (SNRPE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SNRPE Polyclonal antibody (20407-1-AP)

    IHC staining of mouse brain using 20407-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20407-1-AP (SNRPE antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using SNRPE Polyclonal antibody (20407-1-AP)

    IHC staining of human hepatocirrhosis using 20407-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20407-1-AP (SNRPE antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using SNRPE Polyclonal antibody (20407-1-AP)

    IHC staining of human hepatocirrhosis using 20407-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20407-1-AP (SNRPE antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SNRPE Polyclonal antibody (20407-1-AP)

    IHC staining of mouse brain using 20407-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20407-1-AP (SNRPE antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Positive WB detected inmouse placenta tissue, human placenta tissue, human brain tissue
Positive IHC detected inmouse brain tissue, human hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20407-1-AP targets SNRPE in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SNRPE fusion protein Ag14227 相同性解析による交差性が予測される生物種
Full Name small nuclear ribonucleoprotein polypeptide E
Calculated molecular weight 92 aa, 11 kDa
Observed molecular weight 11 kDa
GenBank accession numberBC002639
Gene symbol SNRPE
Gene ID (NCBI) 6635
RRIDAB_10699882
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The snRNP E (SNRPE)protein is one of several proteins associated with the U family of small nuclear RNAs that are involved in RNA processing. It functions in the U7 snRNP complex that is involved in histone 3'-end processing and also associated with snRNP U1, U2, U4/U6 and U5. This antibody raises against the full length of human SNRPE, and can recognize two bands of SNRPE, include a 9 kDa band(isoform) and a 11kDa band(SNRPE)

Protocols

Product Specific Protocols
WB protocol for SNRPE antibody 20407-1-APDownload protocol
IHC protocol for SNRPE antibody 20407-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Am J Hum Genet

Mutations in SNRPE, which encodes a core protein of the spliceosome, cause autosomal-dominant hypotrichosis simplex.

Authors - Sandra M Pasternack
View Article
humanWB,IHC

J Pathol

A novel protein-based prognostic signature improves risk stratification to guide clinical management in early-stage lung adenocarcinoma patients.

Authors - Elena Martínez-Terroba
View Article