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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SNRPF Polyclonal antibody

SNRPF Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14977-1-AP

Synonyms

snRNP F, Sm-F, SMF, Sm protein F, Sm F

Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using SNRPF Polyclonal antibody (14977-1-AP)

    WB analysis of human heart using 14977-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 14977-1-AP (SNRPF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SNRPF Polyclonal antibody (14977-1-AP)

    WB analysis of HeLa using 14977-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14977-1-AP (SNRPF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using SNRPF Polyclonal antibody (14977-1-AP)

    WB analysis of MCF-7 using 14977-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 14977-1-AP (SNRPF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using SNRPF Polyclonal antibody (14977-1-AP)

    WB analysis of mouse kidney using 14977-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14977-1-AP (SNRPF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using SNRPF Polyclonal antibody (14977-1-AP)

    WB analysis of mouse liver using 14977-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 14977-1-AP (SNRPF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using SNRPF Polyclonal antibody (14977-1-AP)

    WB analysis of mouse liver using 14977-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 14977-1-AP (SNRPF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using SNRPF Polyclonal antibody (14977-1-AP)

    WB analysis of HepG2 using 14977-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14977-1-AP (SNRPF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SNRPF Polyclonal antibody (14977-1-AP)

    IHC staining of human breast cancer using 14977-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 14977-1-AP (SNRPF antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SNRPF Polyclonal antibody (14977-1-AP)

    IHC staining of human breast cancer using 14977-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 14977-1-AP (SNRPF antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SNRPF Polyclonal antibody (14977-1-AP)

    IHC staining of human breast cancer using 14977-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 14977-1-AP (SNRPF antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SNRPF Polyclonal antibody (14977-1-AP)

    IHC staining of human breast cancer using 14977-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 14977-1-AP (SNRPF antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SNRPF Polyclonal antibody (14977-1-AP)

    IF Staining of HeLa using 14977-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 14977-1-AP (SNRPF antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inhuman heart tissue, HeLa cells, HepG2 cells, MCF-7 cells, mouse kidney tissue, mouse liver tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14977-1-AP targets SNRPF in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SNRPF fusion protein Ag6889 相同性解析による交差性が予測される生物種
Full Name small nuclear ribonucleoprotein polypeptide F
Calculated molecular weight 10 kDa
Observed molecular weight 10 kDa
GenBank accession numberBC002505
Gene symbol SNRPF
Gene ID (NCBI) 6636
RRIDAB_2302166
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The 4 major small nuclear ribonucleoprotein particles (snRNPs), U1, U2, U4/U6, and U5, are essential components of the eukaryotic pre-mRNA splicing machinery. These snRNPs share 8 proteins which form the snRNP structural core: B/B-prime (SNRPB), D1 (SNRPD1), D2 (SNRPD2), D3 (SNRPD3), E (SNRPE), F (SNRPF), and G (SNRPG). These proteins have a role in the biogenesis of the snRNPs.

Protocols

Product Specific Protocols
WB protocol for SNRPF antibody 14977-1-APDownload protocol
IHC protocol for SNRPF antibody 14977-1-APDownload protocol
IF protocol for SNRPF antibody 14977-1-APDownload protocol
FC protocol for SNRPF antibody 14977-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols