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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SNRPN/SNRPB Monoclonal antibody (67280-1-Ig)

    WB analysis using 67280-1-Ig (same clone as 67280-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67280-1-Ig (SNRPN/SNRPB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67280-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SNRPN/SNRPB Monoclonal antibody (67280-1-Ig)

    IHC staining of human colon cancer using 67280-1-Ig (same clone as 67280-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67280-1-Ig (SNRPN/SNRPB antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67280-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SNRPN/SNRPB Monoclonal antibody (67280-1-Ig)

    IHC staining of human colon cancer using 67280-1-Ig (same clone as 67280-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67280-1-Ig (SNRPN/SNRPB antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67280-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SNRPN/SNRPB Monoclonal antibody (67280-1-Ig)

    IHC staining of human colon cancer using 67280-1-Ig (same clone as 67280-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67280-1-Ig (SNRPN/SNRPB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67280-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SNRPN/SNRPB Monoclonal antibody (67280-1-Ig)

    IHC staining of human colon cancer using 67280-1-Ig (same clone as 67280-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67280-1-Ig (SNRPN/SNRPB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67280-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using SNRPN/SNRPB Monoclonal antibody (67280-1-Ig)

    IF Staining of SH-SY5Y using 67280-1-Ig (same clone as 67280-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using SNRPN/SNRPB antibody (67280-1-Ig, Clone: 1H2F4 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red). This data was developed using the same antibody clone with 67280-1-PBS in a different storage buffer formulation.

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SNRPN/SNRPB Monoclonal antibody, PBS Only

SNRPN/SNRPB Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67280-1-PBS
Clone No.1H2F4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

SNRPN, SMN, Sm-D, Small nuclear ribonucleoprotein-associated protein N, SM D

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67280-1-PBS targets SNRPN/SNRPB in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SNRPN/SNRPB fusion protein Ag14030 相同性解析による交差性が予測される生物種
Full Name small nuclear ribonucleoprotein polypeptide N
Calculated molecular weight 240 aa, 25 kDa
Observed molecular weight 27-29 kDa
GenBank accession numberBC000611
Gene Symbol SNRPN
Gene ID (NCBI) 6638
RRIDAB_2882548
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP63162
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

SNRPN, or SNURF-SNRPN, is a bicistronic imprinted gene that encodes 2 polypeptides, the SmN splicing factor, which is involved in RNA processing, and the SNRPN upstream reading frame (SNURF) polypeptide.The SNRPN gene is transcribed exclusively from the paternally inherited chromosome and shows highest expression in brain and heart. SNRPN may play a role in etiology of Prader-Willi syndrome. This antibody recognizes SNRPN and SNRPB due to high amino acid sequence homology.