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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using SNTB1-Specific Recombinant antibody (84195-1-RR)

    WB analysis of HepG2 using 84195-1-RR

    WB result of SNTB1-Specific antibody (84195-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SNTB1-Specific transfected HepG2 cells.

  • Western Blot (WB) analysis of various lysates using SNTB1-Specific Recombinant antibody (84195-1-RR)

    WB analysis using 84195-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84195-1-RR (SNTB1-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using SNTB1-Specific Recombinant antibody (84195-1-RR)

    IP experiment of HepG2 using 84195-1-RR

    IP result of anti-SNTB1-Specific (IP:84195-1-RR, 4ug; Detection:84195-1-RR 1:600) with HepG2 cells lysate 2000 ug.

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  • KD/KO Validated

SNTB1-Specific Recombinant antibody

SNTB1-Specific Uni-rAbTM Recombinant Antibody for WB, IP, ELISA
Cat No. 84195-1-RR
Clone No.241184F12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Synonyms

SNTB1, SNT2B1, BSYN2, Beta-1-syntrophin, 59 kDa dystrophin-associated protein A1 basic component 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, mouse liver tissue
Positive IP detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84195-1-RR targets SNTB1-Specific in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name syntrophin, beta 1 (dystrophin-associated protein A1, 59kDa, basic component 1)
Calculated molecular weight 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberNM_021021
Gene Symbol SNTB1
Gene ID (NCBI) 6641
RRIDAB_3671747
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ13884
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SNTB1, also named as SNT2B1, BSYN2 and TIP-43, belongs to the syntrophin family. SNTB1 is an adapter protein that binds to and probably organizes the subcellular localization of a variety of membrane proteins. It may link various receptors to the actin cytoskeleton and the dystrophin glycoprotein complex. This antibody is specific to SNTB1.

Protocols

Product Specific Protocols
WB protocol for SNTB1-Specific antibody 84195-1-RRDownload protocol
IP protocol for SNTB1-Specific antibody 84195-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols