Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | HEK-293T cells, HeLa cells, HepG2 cells, Jurkat cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human breast cancer tissue, human kidney tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 8 publications below |
| IF | See 3 publications below |
| IP | See 2 publications below |
Product Information
10304-1-AP targets SNX1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SNX1 fusion protein Ag0499 相同性解析による交差性が予測される生物種 |
| Full Name | sorting nexin 1 |
| Calculated molecular weight | 59 kDa |
| Observed molecular weight | 70-75 kDa |
| GenBank accession number | BC000357 |
| Gene Symbol | SNX1 |
| Gene ID (NCBI) | 6642 |
| RRID | AB_2192217 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13596 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Sorting nexin 1 (SNX1, synonyms: SNX1A, MGC8664, HsT17379) is a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This endosomal protein regulates the cell-surface expression of epidermal growth factor receptor. SNX1 also has a role in sorting protease-activated receptor-1 from early endosomes to lysosomes. This protein may form oligomeric complexes with family members.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SNX1 antibody 10304-1-AP | Download protocol |
| IHC protocol for SNX1 antibody 10304-1-AP | Download protocol |
| IP protocol for SNX1 antibody 10304-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Neurosci A critical role for Sorting Nexin 1 in the trafficking of metabotropic glutamate receptors.
| ||
FASEB J Sorting nexin 1 loss results in increased oxidative stress and hypertension.
| ||
Mol Biol Cell Trafficking defects in WASH-knockout fibroblasts originate from collapsed endosomal and lysosomal networks. | ||