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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SNX10 Polyclonal antibody (26727-1-AP)

    WB analysis using 26727-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26727-1-AP (SNX10 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using SNX10 Polyclonal antibody (26727-1-AP)

    IHC staining of human pancreas cancer using 26727-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 26727-1-AP (SNX10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using SNX10 Polyclonal antibody (26727-1-AP)

    IHC staining of human pancreas cancer using 26727-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 26727-1-AP (SNX10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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SNX10 Polyclonal antibody

SNX10 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26727-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Sorting nexin-10, sorting nexin 10

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26727-1-AP targets SNX10 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SNX10 fusion protein Ag24977 相同性解析による交差性が予測される生物種
Full Name sorting nexin 10
Calculated molecular weight 201 aa, 24 kDa
Observed molecular weight25 kDa
GenBank accession numberBC034992
Gene Symbol SNX10
Gene ID (NCBI) 29887
RRIDAB_3085897
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y5X0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sorting nexins are a diverse group of cytoplasmic and membrane-associated proteins classified by the presence of a phospholipid-binding motif PX domain (PMID:12461558). They are involved in endocytosis and protein trafficking. Mutations in SNX10 (Sorting nexin-10) have been found to account for approximately 4% of all human autosomal recessive osteopetrosis (ARO) that is a genetically heterogeneous disorder caused by reduced bone resorption by osteoclasts (PMID: 23280965; PMID: 28592808).

Protocols

Product Specific Protocols
WB protocol for SNX10 antibody 26727-1-APDownload protocol
IHC protocol for SNX10 antibody 26727-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols