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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SNX15 Monoclonal antibody

SNX15 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1A10B7

Cat no : 68239-1-Ig

Synonyms

Sorting nexin-15, sorting nexin 15, HSAF001435, 1A10B7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SNX15 Monoclonal antibody (68239-1-Ig)

    WB analysis using 68239-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68239-1-Ig (SNX15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SNX15 Monoclonal antibody (68239-1-Ig)

    IHC staining of human kidney using 68239-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68239-1-Ig (SNX15 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using SNX15 Monoclonal antibody (68239-1-Ig)

    IHC staining of human kidney using 68239-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68239-1-Ig (SNX15 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using SNX15 Monoclonal antibody (68239-1-Ig)

    IF Staining of U2OS using 68239-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SNX15 antibody (68239-1-Ig, Clone: 1A10B7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68239-1-Ig targets SNX15 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SNX15 fusion protein Ag8839 相同性解析による交差性が予測される生物種
Full Name sorting nexin 15
Calculated molecular weight 342 aa, 38 kDa
Observed molecular weight 40-50 kDa
GenBank accession numberBC009897
Gene symbol SNX15
Gene ID (NCBI) 29907
RRIDAB_2935326
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SNX15 belongs to the sorting nexin family and is involved in several stages of intracellular trafficking. Overexpression of SNX15 results in a decrease in the processing of insulin and hepatocyte growth factor receptors to their mature subunits (PMID: 11085978). SNX15 is widely expressed, with the highest expression in skeletal muscle, heart, brain, kidney, spleen, thymus, and small intestine.

Protocols

Product Specific Protocols
WB protocol for SNX15 antibody 68239-1-IgDownload protocol
IHC protocol for SNX15 antibody 68239-1-IgDownload protocol
IF protocol for SNX15 antibody 68239-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols