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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SNX16 Polyclonal antibody

SNX16 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13492-1-AP

Synonyms

Sorting nexin-16, sorting nexin 16

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SNX16 Polyclonal antibody (13492-1-AP)

    WB analysis using 13492-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13492-1-AP (SNX16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using SNX16 Polyclonal antibody (13492-1-AP)

    WB analysis of Raji using 13492-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 13492-1-AP (SNX16 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HT-29 cells using SNX16 Polyclonal antibody (13492-1-AP)

    IF Staining of HT-29 using 13492-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using SNX16 antibody (13492-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inJurkat cells, Raji cells, U-251 cells
Positive IF/ICC detected inHT-29 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13492-1-AP targets SNX16 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SNX16 fusion protein Ag4315 相同性解析による交差性が予測される生物種
Full Name sorting nexin 16
Calculated molecular weight 344 aa, 39 kDa
Observed molecular weight39-50 kDa
GenBank accession numberBC033630
Gene symbol SNX16
Gene ID (NCBI) 64089
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SNX16,Sorting nexin 16, is a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which mediates membrane association by interaction with phosphoinositides. SNX16 associates with late endosome membranes as is involved in tubule formation, cholesterol transport, and transport of tetraspanin CD81. It also inhibits cell migration and tumorigenesis. SNX16 directs the sorting of EGFR to the endosomal compartment and thus regulates EGF-induced cell signaling (PMID:15292396). It may function in the trafficking of proteins between the early and late endosomal compartments. SNX16 was detected 49-53 kDa in human (PMID:12813048).

Protocols

Product Specific Protocols
WB protocol for SNX16 antibody 13492-1-APDownload protocol
IF protocol for SNX16 antibody 13492-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols