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- KD/KO Validated
SNX17 Polyclonal antibody
SNX17 Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, IP, ELISA
Conjugate
Unconjugated
Cat no : 10275-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, mouse cerebellum tissue, human brain tissue, mouse skin tissue, mouse brain tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 9 publications below |
| WB | See 18 publications below |
| IF | See 3 publications below |
| IP | See 2 publications below |
Product Information
10275-1-AP targets SNX17 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SNX17 fusion protein Ag0355 相同性解析による交差性が予測される生物種 |
| Full Name | sorting nexin 17 |
| Calculated molecular weight | 53 kDa |
| Observed molecular weight | 50 kDa |
| GenBank accession number | BC002524 |
| Gene symbol | SNX17 |
| Gene ID (NCBI) | 9784 |
| RRID | AB_2192394 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Sorting nexin 17 (SNX17, synonym: KIAA0064) is a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. SNX17 does not contain a coiled-coil region, like some family members, but contains a B41 domain. This protein interacts with the cytoplasmic domain of P-selectin and may function in the intracellular trafficking of P-selectin.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SNX17 antibody 10275-1-AP | Download protocol |
| IHC protocol for SNX17 antibody 10275-1-AP | Download protocol |
| IP protocol for SNX17 antibody 10275-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Sorting nexin 17 prevents lysosomal degradation of β1 integrins by binding to the β1-integrin tail.
| ||
Nat Cell Biol Retriever is a multiprotein complex for retromer-independent endosomal cargo recycling. | ||
Nat Cell Biol Recycling of autophagosomal components from autolysosomes by the recycler complex. | ||
Nat Commun A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry. | ||
Proc Natl Acad Sci U S A LRP1 is a receptor for Clostridium perfringens TpeL toxin indicating a two-receptor model of clostridial glycosylating toxins.
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