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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SNX17 Monoclonal antibody (68256-1-Ig)

    WB analysis using 68256-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68256-1-Ig (SNX17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SNX17 Monoclonal antibody (68256-1-Ig)

    WB analysis using 68256-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68256-1-Ig (SNX17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using SNX17 Monoclonal antibody (68256-1-Ig)

    WB analysis of Jurkat using 68256-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 68256-1-Ig (SNX17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SNX17 Monoclonal antibody (68256-1-Ig)

    IF Staining of HepG2 using 68256-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SNX17 antibody (68256-1-Ig, Clone: 3B8F6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SNX17 Monoclonal antibody (68256-1-Ig)

    IF Staining of HeLa using 68256-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using SNX17 antibody (68256-1-Ig, Clone: 3B8F6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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SNX17 Monoclonal antibody

SNX17 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68256-1-Ig
Clone No.3B8F6

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, pig

Applications

WB, IF/ICC, ELISA

Synonyms

KIAA0064, SNX17, sorting nexin 17

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, pig brain tissue, Jurkat cells, rat brain tissue, HeLa cells
Positive IF/ICC detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68256-1-Ig targets SNX17 in WB, IF/ICC, ELISA applications and shows reactivity with Human, rat, pig samples.

Tested Reactivity Human, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SNX17 fusion protein Ag32624 相同性解析による交差性が予測される生物種
Full Name sorting nexin 17
Calculated molecular weight 53 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC002524
Gene Symbol SNX17
Gene ID (NCBI) 9784
RRIDAB_2935341
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ15036
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sorting nexin 17 (SNX17, synonym: KIAA0064) is a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. SNX17 does not contain a coiled coil region, like some family members, but contains a B41 domain. This protein interacts with the cytoplasmic domain of P-selectin, and may function in the intracellular trafficking of P-selectin.

Protocols

Product Specific Protocols
WB protocol for SNX17 antibody 68256-1-IgDownload protocol
IF protocol for SNX17 antibody 68256-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols