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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SNX20 Polyclonal antibody

SNX20 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13180-1-AP

Synonyms

SLIC 1, SLIC1, SNX20, sorting nexin 20

Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using SNX20 Polyclonal antibody (13180-1-AP)

    WB analysis of COLO 320 using 13180-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 13180-1-AP (SNX20 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using SNX20 Polyclonal antibody (13180-1-AP)

    WB analysis of mouse thymus using 13180-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13180-1-AP (SNX20 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of COLO 320 cells using SNX20 Polyclonal antibody (13180-1-AP)

    IP experiment of COLO 320 using 13180-1-AP

    IP result of anti-SNX20 (IP:13180-1-AP, 4ug; Detection:13180-1-AP 1:300) with COLO 320 cells lysate 2000ug.

  • Immunohistochemistry (IHC) staining of human placenta tissue using SNX20 Polyclonal antibody (13180-1-AP)

    IHC staining of human placenta using 13180-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 13180-1-AP (SNX20 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using SNX20 Polyclonal antibody (13180-1-AP)

    IHC staining of human placenta using 13180-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 13180-1-AP (SNX20 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using SNX20 Polyclonal antibody (13180-1-AP)

    IHC staining of human lung using 13180-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 13180-1-AP (SNX20 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using SNX20 Polyclonal antibody (13180-1-AP)

    IHC staining of human lung using 13180-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 13180-1-AP (SNX20 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inCOLO 320 cells, mouse thymus tissue
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman placenta tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13180-1-AP targets SNX20 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SNX20 fusion protein Ag3847 相同性解析による交差性が予測される生物種
Full Name sorting nexin 20
Calculated molecular weight 316 aa, 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC027944
Gene symbol SNX20
Gene ID (NCBI) 124460
RRIDAB_10638911
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SNX20 antibody 13180-1-APDownload protocol
IHC protocol for SNX20 antibody 13180-1-APDownload protocol
IP protocol for SNX20 antibody 13180-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols